We narrowed to 18,349 results for: MUT

1. FLAG-JAK1Gly590Arg

   Plasmid

   #174573

   Purpose

   Human FLAG-tagged JAK1 Gly590Arg mutant expression (N-term. tag) (CMV prom.)

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   JAK1Gly590Arg
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Gly590Arg

   Promoter

   CMV

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEX-SaCas9-U6-sgGria3

   Plasmid

   #124855

   Purpose

   Mutagenesis of Gria3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria3 (Gria3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-Nkx2-1*/Neo

   Plasmid

   #31272

   Depositor

   Tyler Jacks, Monte Winslow

   Article

   Winslow et al Nature. 2011 May 5. 473(7345):101-4.

   Insert

   Nkx2-1* (Nkx2-1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   A shNkx2-1 insensitive Nkx2-1 cDNA was created by…

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-RaichuEV-Rac1(T17N)

   Plasmid

   #164902

   Purpose

   Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   RaichuEV-Rac1 (RAC1 Human)
   

   Expression

   Mammalian

   Mutation

   T17N mutation in the Rac1 domain which make the s…

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MLM3720

   Plasmid

   #49944

   Purpose

   Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x FLAG Tet1CDmut RH-3 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Mutation

   catalytic domain of TET1 inactive (H1671Y and D16…

   Promoter

   EF1a

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-mCherry-TSC2(AA,3Q)

   Plasmid

   #192444

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-Blast SHMT2 CD

   Plasmid

   #106302

   Purpose

   Expresses SHMT2 K280A catalytic site mutant

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 2 (SHMT2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   K280A catalytic site mutation, Silent mutations d…

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNUT N6His K206E/K534A hTFNG

   Plasmid

   #70133

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe

   Depositor

   Anne Mason, Ross MacGillivray

   Article

   Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.

   Insert

   N6His K206e/K534A hTFNG (TF Human)
   

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   changed Lys206 to a glutamic acid and Lys534 to a…

   Promoter

   SV40

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST53-LRRK2-K1347A

   Plasmid

   #25052

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 1347 mutated to Alanine

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pProEx-HTa-SOD1 C57D/C146D

    Plasmid

    #191851

    Purpose

    express C57D/C146D mutant SOD1 in bacterial cells

    Depositor

    Nam-Chul Ha

    Article

    Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Bacterial

    Mutation

    C57D, C146D

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MLM3762

    Plasmid

    #49947

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x FLAG Tet1CDmut RH-4 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

    Plasmid

    #107163

    Purpose

    Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme

    Depositor

    Jose Carlos Cameselle

    Article

    Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.

    Insert

    F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. ORAI1-YFP T184M

    Plasmid

    #114181

    Purpose

    ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).

    Depositor

    Nicolas Demaurex

    Article

    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

    Insert

    ORAI1-YFP T184M (ORAI1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    T184M

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-PTBP1-RRM12CCtoSS

    Plasmid

    #89155

    Purpose

    Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
    

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMV-cGAS R71/75E C396/7A-HA

    Plasmid

    #130920

    Purpose

    Expresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    human cGAS R71/75E C396/7A
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 71 and 75 mutated to glutamate; aa 396 and 397…

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEXfrt-SaCas9-U6-sgGria3

    Plasmid

    #124869

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.2-Cx43-ML-HA

    Plasmid

    #49857

    Purpose

    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    All internal Methionines mutated to Leucine. Cont…

    Promoter

    CMV

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCEP4-CCR5(R5gp120-Hi)

    Plasmid

    #98954

    Purpose

    Mammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-Hi

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    R5gp120-Hi variant: P8L, I9E, I12M, S17E, P19D, Q…

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5 TO FRT Myc ArB K106R

    Plasmid

    #59807

    Purpose

    Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora B (AURKB Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K106R, kinase mutant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. HNF4A_P2-285/-192

    Plasmid

    #31053

    Depositor

    Gerhart Ryffel

    Article

    Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    -285 to -1 of P2 HNF4A promoter with mutation -19…

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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