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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKIBra-P2A-mCherry-Puro-sfGFP

Plasmid

#115686

Purpose

Knock-in of P2A-mCherry at the mouse Brachyury (T) gene

Depositor

David Suter

Publication

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

P2A-mCherry-loxP-PGK-Puro-sfGFP-loxP

Expression

Mammalian

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTXRE5-Z

Plasmid

#112509

Purpose

Reporter plasmid, responsive to activated Ah receptor/Arnt

Depositor

Charles Miller

Publication

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

RW95-3 with 5x xenobiotic response elements (lacZ Synthetic, E.coli)

Expression

Yeast

Promoter

5 XREs+ minimal Cytochrome C

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGM202T7

Plasmid

#69993

Purpose

E. coli/Streptomyces expression vector containing the T7-promoter

Depositor

Günther Muth

Publication

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aphII
tsr
rep
sso
T7 promoter
dso
His-Orf

Tags

N-terminal and C-terminal His tag encoding sequen…

Expression

Bacterial

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDisplay-pHoran4

Plasmid

#61557

Purpose

Orange fluorescent protein based pH indicator in pDisplay vector

Depositor

Robert Campbell

Publication

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHoran4

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pUC-UAS1B28-Leum

Plasmid

#44327

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B28-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B28-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pLV.SP146.gp91.eGFP.cHS4

Plasmid

#30476

Depositor

Didier Trono

Publication

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/eGFP/cHS4

Use

Lentiviral

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST_FUS_C2G_R521G

Plasmid

#26380

Depositor

Thomas Tuschl

Publication

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

R521G

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 Y42A/M43A

Plasmid

#19940

Depositor

Yue Xiong

Publication

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 Y42A/M43A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a SKP1 binding mutant

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
HOXB4 (human) HA-TAT-tag pET

Plasmid

#8526

Depositor

Corey Largman

Publication

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

HA

Expression

Bacterial

Mutation

replace T7 and HIS tags of pET with an HA tag;

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/GFP-LL5BIP

Plasmid

#112829

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Publication

Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

Insert

LL5BIP (2410002F23Rik Mouse)

Tags

GFP

Expression

Mammalian

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LifeAct-mhYFP

Plasmid

#186524

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to actin

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag3HuR

Plasmid

#135950

Purpose

Expresses HuR in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Publication

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Tags

Flag3

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21ΔRING-Box

Plasmid

#105523

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLifeAct_mScarlet-H_N1

Plasmid

#85055

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-PAT1

Plasmid

#88856

Purpose

myc-TAG overexpression

Depositor

David Sabatini

Publication

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

SLC36A1 (SLC36A1 Human)

Tags

myc

Expression

Mammalian

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTC147

Plasmid

#70013

Purpose

T-DNA vector with Kanamycin resistance carrying a 35S:ANT1 over-expression cassette.

Depositor

Daniel Voytas

Publication

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

35S:ANT1

Expression

Plant

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pQC

Plasmid

#48100

Purpose

Cumate-inducible expression of ECFP-tagged fusion protein

Depositor

Julia Vorholt

Publication

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG and ECFP

Expression

Bacterial

Promoter

PQ5

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179117

Purpose

Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Publication

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Fermt2 sgRNA

Use

AAV

Promoter

U6

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-60nmSAH-GFP(1-10)

Plasmid

#61663

Purpose

2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Publication

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-60nmSAH-GFP(1-10)

Expression

Mammalian

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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