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pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

Plasmid

#100855

Purpose

AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-jYCaMP1s

Plasmid

#135424

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has Service

AAV1

Insert

jYCaMP1s

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-jYCaMP1s

Plasmid

#135420

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has Service

AAV1

Insert

jYCaMP1s

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-Flpo

Plasmid

#174378

Purpose

also known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Has Service

AAV Retrograde

Insert

FLPo recombinase

Use

AAV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDblet

Plasmid

#8848

Depositor

Joel Huberman

Article

Brun et al Gene. 1995 Oct 16. 164(1):173-7.

Insert

ARS doublet

Use

Yeast cloning vector

Expression

Yeast

Mutation

This vector contains, between AatII sites, a dire…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pTorPE-B-GECO1

Plasmid

#32469

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pDJSVT86

Plasmid

#108965

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

Depositor

Daniel Slade

Article

Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

Type

Empty backbone

Tags

OmpA 1-27, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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RepX_pET28a(+)

Plasmid

#172199

Purpose

Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

Depositor

Taekjip Ha

Article

Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

Insert

RepX superhelicase

Tags

6xHis tag

Expression

Bacterial

Mutation

C18L, C43S, C167V, S400C, C612A

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHCMV_GP64

Plasmid

#207276

Purpose

Mammalian expression of baculovirus GP64 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

GP64

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO ChRmine-eYFP-WPRE

Plasmid

#130996

Purpose

Double floxed ChRmine-eYFP under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Promoter

Ef1a

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin-S3A

Plasmid

#78296

Purpose

Mammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFP

Depositor

Michael Frotscher

Article

Chai et al Development. 2016 Mar 15;143(6):1029-40.

Insert

cofilin 1 (Cfl1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

S3A

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShHELIX-KanR (CJT70)

Plasmid

#181793

Purpose

pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-Rncp-iGluSnFR1

Plasmid

#107336

Purpose

Red fluorescent glutamate sensor with ncp topology. Membrane-displayed

Depositor

Robert Campbell

Article

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Insert

Rncp-iGluSnFR1

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33Y)-pcw107-V5

Plasmid

#64616

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral

Tags

V5

Mutation

S33Y

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

Plasmid

#162388

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Tags

6xHis

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138133

Purpose

Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pF148 pSOD1G37RAcGFP1

Plasmid

#26409

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G37R

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138128

Purpose

Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1a-MICB*005-IRES-ZsGreen

Plasmid

#114008

Purpose

Induces expression of human MICB allele 005 cDNA

Depositor

Kai Wucherpfennig

Article

Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

Insert

MICB*005 (MICB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-GR-GECO1.2

Plasmid

#42187

Depositor

Robert Campbell

Article

Hoi et al J Am Chem Soc. 2012 Dec 26.

Insert

GR-GECO1.2

Promoter

CMV

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only