We narrowed to 6,263 results for: wus

1. pDSM-bc-Prps3-CAD-Tcyc1

   Plasmid

   #127728

   Purpose

   Yeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   cis-aconitate decarboxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Prps3

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSM-bc-Peft2-CAD-Tklura3

   Plasmid

   #127729

   Purpose

   Yeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   cis-aconitate decarboxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Peft2

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDSM-bc-Psmtef1-CAD-Tyhi9

   Plasmid

   #127730

   Purpose

   Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   cis-aconitate decarboxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Psmtef1

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDSM-cd-Psptdh3-ACO-Tagtef1

   Plasmid

   #127734

   Purpose

   Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   aconitase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Psptdh3

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDSM-de-Ppgk1-PYC-Ttpi1

   Plasmid

   #127735

   Purpose

   Yeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Ppgk1

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDSM-de-Ppxr1-PYC-Tnat1

   Plasmid

   #127736

   Purpose

   Yeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Ppxr1

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDSM-ef-Psbtdh3-CSC-Tefm1

   Plasmid

   #127742

   Purpose

   Yeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   citrate synthase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Psbtdh3

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDSM-f3-Peno2-ACDH-Ttal1

   Plasmid

   #127743

   Purpose

   Yeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   acetylating acetaldehyde dehydrogenase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Peno2

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDSM-f3-Pvma6-ACDH-Trps14a

   Plasmid

   #127744

   Purpose

   Yeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   acetylating acetaldehyde dehydrogenase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pvma6

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDSM-f3-Prpl28-ACDH-Tecm10

    Plasmid

    #127745

    Purpose

    Yeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    acetylating acetaldehyde dehydrogenase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Prpl28

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDSM-f3-Ptdh3-ACDH-Trpl3

    Plasmid

    #127746

    Purpose

    Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    acetylating acetaldehyde dehydrogenase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ptdh3

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCmini-iCAP-PHP.eB

    Plasmid

    #103005

    Purpose

    non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    Synthetic construct isolate AAV-PHP.eB VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid

    #170186

    Purpose

    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

    Depositor

    Bradley Bernstein

    Article

    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

    Insert

    CreERT2-T2A-eBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR_DKO

    Plasmid

    #183192

    Purpose

    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, mU6

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Vamp2-pHluorin

    Plasmid

    #190151

    Purpose

    Expresses (pH-sensitive) super ecliptic pHluorin-tagged mouse/rat Vamp2 for imaging exocytosis

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_LMX1A_P2A_Hygro_Barcode

    Plasmid

    #120457

    Purpose

    Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    LMX1A (LMX1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuide_DKO

    Plasmid

    #183193

    Purpose

    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, mU6

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. rAAV2/MG1.2

    Plasmid

    #184541

    Purpose

    AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

    Insert

    AAV-MG1.2 capsid
    

    Use

    AAV; Helper vector for packaging

    Tags

    Expression

    Mutation

    changes on AAV9 capsid: K449R, Q588T; insertion o…

    Promoter

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRL CAGpNFlag BAF155 ires GFP

    Plasmid

    #24561

    Depositor

    Jerry Crabtree

    Article

    Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

    Insert

    BAF155 (Smarcc1 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available Since

    July 16, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pMV-LEU2

    Plasmid

    #65334

    Purpose

    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    LEU2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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