We narrowed to 17,819 results for: Mut

1. pET21a Hp-iPGM-bio

   Plasmid

   #179615

   Purpose

   Expresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coli

   Depositor

   James Inglese

   Article

   van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

   Insert

   H. pylori iPGM bio-6His
   

   Use

   Tags

   His tag and biotinylation sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

   Plasmid

   #153445

   Purpose

   E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   6xHis and ybbr tag

   Expression

   Bacterial

   Mutation

   Serine 199 was mutated to amber codon (TAG). Argi…

   Promoter

   T7 promoter

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

   Plasmid

   #153444

   Purpose

   E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   6xHis and ybbr tag

   Expression

   Bacterial

   Mutation

   Serine 199 was mutated to amber codon (TAG). Argi…

   Promoter

   T7 promoter

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUG-T2A-F241S

   Plasmid

   #140740

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation F241S and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTENF241S (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   F241S

   Promoter

   hUbiC

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUG-T2A-W274L

   Plasmid

   #140742

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation W274L and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTENW274L (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   W274L

   Promoter

   hUbiC

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUG-T2A-D252G

   Plasmid

   #140741

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation D252G and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTEND252G (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   D252G

   Promoter

   hUbiC

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUSEss-CHIg-mG3_CH2charge

   Plasmid

   #105858

   Purpose

   Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG3 heavy chain (Ighg3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutated heavy chain of mouse IgG3, muatations: Hi…

   Promoter

   hEF1-HTLV prom

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MBP-hnRNPA2_LC_R191K_R254K

   Plasmid

   #104466

   Purpose

   express MBP hnRNPA2 LC with 2 R to K mutations

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   R191K R254K

   Promoter

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJC20cdc8.27

   Plasmid

   #99362

   Purpose

   Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Glutamic acid 129 to Lysine

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500

    Plasmid

    #92315

    Purpose

    Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    B1 domain of protein L (PL) mutant K16TAG
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70b (p70a promoter with one mutation)

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMCh-660

    Plasmid

    #92259

    Purpose

    Plasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    CMV

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCh-665

    Plasmid

    #92254

    Purpose

    Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    EF1A

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST47-ARL4D

    Plasmid

    #67468

    Purpose

    Mammalian expression of hArl4D with stop codon (native expression)

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pDEST40-ARL4D-V5-His6

    Plasmid

    #67447

    Purpose

    Mammalian expression of hArl4D with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pET-DEST42-ARL4D-V5-His6

    Plasmid

    #67426

    Purpose

    Expression of hArl4D with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pDEST47-ARL4D-GFP

    Plasmid

    #67405

    Purpose

    Mammalian expression of hArl4D with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDONR221-ARL4D-no stop

    Plasmid

    #67337

    Purpose

    hArl4D without stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    None

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA-HA-ER D538G

    Plasmid

    #49500

    Purpose

    Expresses HA-tagged ER D538G mutant in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Aspartic acid 538 to Glycine

    Promoter

    CMV

    Available Since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-HA-ER Y537S

    Plasmid

    #49499

    Purpose

    Expresses HA-tagged ER Y537S mutant in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tyrosine 537 to Serine

    Promoter

    CMV

    Available Since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-FLAG-hSF3B1-K700E

    Plasmid

    #82577

    Purpose

    Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

    Depositor

    Manoj Pillai

    Article

    Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

    Insert

    human SF3B1-K700E (SF3B1 Human)
    

    Use

    Tags

    FLAG peptide

    Expression

    Mammalian

    Mutation

    K700E

    Promoter

    CMV

    Available Since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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