We narrowed to 22,342 results for: Sele

1. pCEP4-myc-EphB4

   Plasmid

   #200983

   Purpose

   Mammalian expression plasmid for myc-tagged EphB4

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB4 (EPHB4 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-HeFSpCas9-NLS-6xHis

   Plasmid

   #207391

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-B-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207378

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-Blackjack SpCas9-NLS-6xHis

   Plasmid

   #207375

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Blackjack SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 1005-1013 replaced with two glycine

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IRES-puro-3xFLAG

   Plasmid

   #204545

   Purpose

   Expresses 3xFLAG tag in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   3xFlag
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P-1-MVP

   Plasmid

   #204541

   Purpose

   Expresses GST-tagged MVP in E.coli

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   MVP (MVP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28a(+)-6xHis-TEV-ORF9c

   Plasmid

   #201023

   Purpose

   Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   open reading frame 9c
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas-mut4/1_2_VB

   Plasmid

   #186444

   Purpose

   Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas4 domain (K81A)

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pnYC-NpH-Hs4EHP_52-234opt_AE

   Plasmid

   #148566

   Purpose

   Bacterial Expression of Hs4EHP_52-234opt

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   Hs4EHP_52-234opt (EIF4E2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnEA-NpM-HsGIGYF1_33-52-GB1_AE

    Plasmid

    #148552

    Purpose

    Bacterial Expression of HsGIGYF1_33-52

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF1_33-52 (GIGYF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pnEA-NpM-HsGIGYF1_33-71_AE

    Plasmid

    #148553

    Purpose

    Bacterial Expression of HsGIGYF1_33-71

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF1_33-71 (GIGYF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pnEA-NpM-HsGIGYF1_33-71-GB1_AE

    Plasmid

    #148554

    Purpose

    Bacterial Expression of HsGIGYF1_33-71

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF1_33-71 (GIGYF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pnEA-NpM-HsGIGYF1_33-103-E44AE45FQ87A_AE

    Plasmid

    #148555

    Purpose

    Bacterial Expression of HsGIGYF1_33-103-E44AE45FQ87A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF1_33-103-E44AE45FQ87A (GIGYF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnEA-NpM-HsGIGYF1_33-103-GB1_AE

    Plasmid

    #148557

    Purpose

    Bacterial Expression of HsGIGYF1_33-103

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF1_33-103 (GIGYF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAGGS-Flex/3'USS(103)-GFP

    Plasmid

    #197890

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAGGS-Flex/3'USS(553)-GFP

    Plasmid

    #197888

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAGGS-Flex/3'USS(285)-GFP

    Plasmid

    #197889

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV(MinDis)-iEscSnFR_PM

    Plasmid

    #182813

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iEscSnFR_PM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV(MinDis)-iEscSnFR_cyto

    Plasmid

    #182814

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iEscSnFR_cyto
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV(MinDis)-iFluoxSnFR_ER

    Plasmid

    #182815

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iFluoxSnFR_ER
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge