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pJPC060

Plasmid

#234535

Purpose

TRV2 expressing FLPe

Depositor

Daniel Voytas

Article

Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

Insert

FLPe recombinase

Use

Recombinase

Expression

Plant

Promoter

CaMV 35S

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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SJZ6-HIS5

Plasmid

#228259

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

his5

Expression

Yeast

Promoter

TEF1 S.c.

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJP_Ctrl10

Plasmid

#219672

Purpose

Contains PT3lacO promoter (regulated by blue light and LacI) controlling the expression of mCherry. LacI is constitutively produced by pR promoter.

Depositor

Yolanda Schaerli

Article

Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

Inserts

mCherry
lacI

Promoter

PT3lacO and pR

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJP_Rep00

Plasmid

#219677

Purpose

Light-inducible repressilator (modified from pLPT107). PT3lacO promoter controls the expression of TetR node.

Depositor

Yolanda Schaerli

Article

Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

Insert

PT3lacO

Use

Synthetic Biology

Promoter

PT3lacO

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-ChmN-C47A

Plasmid

#221367

Purpose

Express C47A ChmN in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmN-C47A

Tags

His6

Expression

Bacterial

Mutation

changed Cys 47 to Ala

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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Sp(AU)sgRNA-mKO2

Plasmid

#184443

Purpose

sgRNA with AU flip (sgRNA2.0), mKO2 as selection marker

Depositor

Rhys Allan

Article

Chan et al Immunol Cell Biol. 2020 Sep 24. doi: 10.

Insert

sgRNA with AU flip, mKO2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMV23

Plasmid

#128887

Purpose

Position 4 module- STOP (stop codon)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

STOP

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMV10

Plasmid

#128892

Purpose

Position 6 module- URA3

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

URA3 (URA3 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV5

Plasmid

#128886

Purpose

Position 4 module- CLN2-PEST (Degron)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

CLN2-PEST

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Rabbit NHERF-1

Plasmid

#31644

Depositor

Edward Weinman

Article

Weinman et al J Clin Invest. 1995 May . 95(5):2143-9.

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Expression

Bacterial

Mutation

none

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO SwiChRca-TS-EYFP

Plasmid

#55631

Purpose

Cre-Dependent Chloride Channel

Depositor

Karl Deisseroth

Article

Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

Insert

iC1C2-TS-EYFP

Use

AAV and Cre/Lox

Tags

EYFP

Expression

Mammalian

Mutation

C128A

Promoter

Ef1a

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagRFP-2A-BSD

Plasmid

#167930

Purpose

Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNM3689

Plasmid

#154111

Purpose

miniMos single component RMCE landing site vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3

Use

Minimos

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCR1265

Plasmid

#111094

Purpose

lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 0

Plasmid

#206991

Purpose

Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Expression

Bacterial, Mammalian, and Y…

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 1

Plasmid

#206992

Purpose

Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 2

Plasmid

#206993

Purpose

Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagBFP-2A-BSD

Plasmid

#167929

Purpose

Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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