We narrowed to 6,087 results for: nls

1. MTK3a_025

   Plasmid

   #123777

   Purpose

   Encodes dspCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   spdCAS9 (with NLS)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. RNAPII_CTD27-52_K5S_S1966C

   Plasmid

   #98683

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966C

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K1838S, K1859S, K1866S, K1873S, K1887S, S1966C

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. STEME-1

   Plasmid

   #138137

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice protoplasts

   Depositor

   Caixia Gao

   Insert

   APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. A3A-PBE-NG

   Plasmid

   #138141

   Purpose

   Targeted C-to-T in rice protoplasts

   Depositor

   Caixia Gao

   Insert

   APOBEC3A-nCas9-NG-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAdx-CMV-YFP

   Plasmid

   #73348

   Purpose

   Express YFP-NLS under CMV promoter

   Depositor

   Kazuhiro Oka

   Insert

   CMV-YFP
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. STEME-2

   Plasmid

   #138138

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice protoplasts

   Depositor

   Caixia Gao

   Insert

   wtTadA-TadA7.10-APOBEC3A-nCas9-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CAG-dCasRx-RBM25-IRES-BleoR

   Plasmid

   #221002

   Purpose

   Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

   Depositor

   Mikko Taipale, Benjamin Blencowe

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   dCasRx-RBM25-IRES-BleoR
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-Puro-miniAAVR-FLAG

   Plasmid

   #166717

   Purpose

   Vector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tag

   Depositor

   Jan Carette

   Article

   Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

   Insert

   miniAAVR-FLAG
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Only expressing PKD1-3 of ectodomain

   Promoter

   CMV

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. KRAB-dCas9

   Plasmid

   #112195

   Purpose

   KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   KRAB (ZNF10 Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-KRAB-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PIEZO1-HaloTag

    Plasmid

    #207834

    Purpose

    PIEZO1 fusion for biochemistry and live imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    PIEZO1 (PIEZO1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NG-ABE8e(V106W)

    Plasmid

    #198533

    Purpose

    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHS_Nova

    Plasmid

    #239831

    Purpose

    NovaIscB mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    NovaIscB
    

    Use

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Insertion of REC domain from NbaCas9-1 and swap o…

    Promoter

    CMV

    Available since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJ922

    Plasmid

    #78312

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Use

    Tags

    HA-2xNLS-EGFP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-ABE7.10

    Plasmid

    #102919

    Purpose

    expresses ABE7.10 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.10
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpRY-ABE8e(V106W)

    Plasmid

    #198553

    Purpose

    Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDRM98 mKate2 Blast

    Plasmid

    #174720

    Purpose

    Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    mKate2
    BSD
    

    Use

    Lentiviral

    Tags

    P2A linker and SV40 NLS with GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND and MND (off mKate-P2A)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL3718 (pQCascade_Pse)

    Plasmid

    #200899

    Purpose

    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCas7, PseCas8, PseCas6, PseTniQ
    

    Use

    Tags

    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9-HF1

    Plasmid

    #138556

    Purpose

    Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. xCas9(3.7)-ABE(7.10)

    Plasmid

    #108382

    Purpose

    Mammalian expression vector for xCas9(3.7)-ABE

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-ABE(7.10)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ223 (dAsCpf1-SRDX)

    Plasmid

    #86209

    Purpose

    Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    dAsCpf1
    

    Use

    Gateway compatible cpf1 vectors

    Tags

    NLS and SRDX repressor and NLS

    Expression

    Plant

    Mutation

    D908A

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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