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pAAV.hSyn.iGluSnFR3.v857.PDGFR

Plasmid

#178329

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Has Service

AAV1

Insert

pAAV hSyn iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-ChR2-mCherry

Plasmid

#137144

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin- soCoChR-GFP

Plasmid

#107708

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Has Service

AAV9

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Promoter

hSynapsin

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLENTI_hACE2_HygR

Plasmid

#155296

Purpose

Lentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance

Depositor

Raffaele De Francesco

Article

Notarbartolo et al Sci Immunol. 2021 Aug 10;6(62). pii: 6/6

Insert

hACE2 (ACE2 Human)

Use

Lentiviral

Tags

C9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_1_Cas9

Plasmid

#233456

Purpose

Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 2 RECKLEEN plasmid

Use

CRISPR

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-EYFP

Plasmid

#35507

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.CaMPARI.WPRE.SV40

Plasmid

#100832

Purpose

AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Has Service

AAV1, AAV5, and AAV9

Insert

CaMPARI

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP

Plasmid

#137141

Purpose

Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChR2(ET/TC)-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E123T, T159C

Promoter

nEF

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-sRGECO

Plasmid

#137127

Purpose

Intersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130991

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

Plasmid

#98927

Purpose

Cre dependent AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1

Insert

GFP-smFP-myc

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-RdLight1

Plasmid

#125708

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

RdLight1

Use

AAV

Tags

Flag tag

Promoter

hSynapsin1

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_OT1.0

Plasmid

#185386

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Has Service

AAV1 and AAV9

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Promoter

hSyn

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE

Plasmid

#179459

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV1

Insert

JEDI-2P-Kv

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1b.WPRE.SV40

Plasmid

#100857

Purpose

AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn-FLEX.iGABASnFR

Plasmid

#112163

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR

Use

AAV

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-sRGECO

Plasmid

#137128

Purpose

Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iAChSnFR

Plasmid

#137955

Purpose

Expresses iAChSnFR under CAG promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Has Service

AAV1

Insert

iAChSnFR

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_nLightG

Plasmid

#187179

Purpose

Expresses green norepinephrine indicator nLightG in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Has Service

AAV9

Insert

nLightG

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_DA-mut

Plasmid

#140555

Purpose

Expresses the DA-insensitive control sensor GRAB_DA-mut in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

DA-insensitive sensor GRAB_DA-mut (control sensor)

Use

AAV

Promoter

hSyn

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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