Skip to main content
Addgene

We narrowed to 12,322 results for: BASE

Showing: 5801 - 5840 of 12322 results
  1. TRE reporter backbone

    Plasmid
    #158678
    Purpose
    This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    mStrawberry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pBECKP-km

    Plasmid
    #117235
    Purpose
    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae
    Depositor
    Insert
    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. iRFP-H2A

    Plasmid
    #158691
    Purpose
    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers
    Depositor
    Insert
    iRFP670
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pEM-Cas9HF1

    Plasmid
    #89961
    Purpose
    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
    Depositor
    Insert
    Cas9HF1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N497A/R661A/Q695A/Q926A
    Promoter
    pTet
    Available since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. PatoL-OG241

    Plasmid
    #72841
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.
    Depositor
    Insert
    PatoL
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoL
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pDV06.2c_DART-VADAR_iRFP720-sensor

    Plasmid
    #200410
    Purpose
    pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2
    Depositor
    Insert
    Closed-loop DART VADAR sensor detecting iRFP720
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid
    #83239
    Purpose
    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.
    Depositor
    Insert
    roGFP2-Tsa2dCpdCr
    Use
    Tags
    roGFP2 fusion with Tsa2dCpdCr
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
  9. pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS

    Plasmid
    #199354
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    TagBFP-P2A-ALFA-SUMOEu
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 5801 - 5840 of 12322 results