We narrowed to 4,223 results for: 424

1. pLD-puro-Cn-VA-NHEJ1-WT

   Plasmid

   #141339

   Purpose

   Lentiviral vector for expression of human NHEJ1-WT in mammalian cells

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   NHEJ1 (NHEJ1 Human)
   

   Use

   Lentiviral

   Tags

   VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTWIN1-His6-Ssp-Httex1-43Q

   Plasmid

   #84352

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 43Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUltra-Exon1Q145-Myc-B

   Plasmid

   #110489

   Purpose

   Lentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)

   Depositor

   Baoji Xu

   Article

   Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

   Insert

   Homo sapiens huntingtin (HTT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   partial long 3'UTR wild type huntingtin exon…

   Promoter

   UbC

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastbac-Dual-StrepII-BARD1

   Plasmid

   #137166

   Purpose

   Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   BARD1 (BARD1 Human)
   

   Use

   Tags

   StrepII

   Expression

   Insect

   Mutation

   fully synthetic, codon optimized for insect expre…

   Promoter

   p10

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF1083

   Plasmid

   #143922

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FUBP1 (FUBP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-DNER

   Plasmid

   #51053

   Purpose

   expression of HA-DNER in mammalian cells

   Depositor

   Mineko Kengaku

   Article

   Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

   Insert

   DNER (Dner Mouse)
   

   Use

   Tags

   HA and signal peptide

   Expression

   Mammalian

   Mutation

   without endogenous signal peptide (1-221bp)

   Promoter

   CMV

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-C2m2-SNAP

   Plasmid

   #208791

   Purpose

   For AAV production to express C2m2-SNAP in astrocytes

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-SNAP (Mfge8 Mouse)
   

   Use

   AAV

   Tags

   SNAP-tag

   Expression

   Mammalian

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   GFAP promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)

   Plasmid

   #174630

   Purpose

   Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Use

   Tags

   GFP-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGex-4T-2_LC3A

   Plasmid

   #73946

   Purpose

   For recombinant expression of human LC3A/MAP1LC3A in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   LC3A (MAP1LC3A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

10. GFP-ataxin-3 C14A

    Plasmid

    #89977

    Purpose

    Expresses catalytic inactive GFP-tagged ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 14 to Alanine

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5FRT/TO-FAF1-Strep-HA

    Plasmid

    #113486

    Purpose

    Expression of human FAF1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    FAF1 (FAF1 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-C-Solo-mStayGold-CLTC

    Plasmid

    #227313

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-MAP3K2

    Plasmid

    #85652

    Purpose

    retroviral expression of human MAP3K2

    Depositor

    Julien Sage

    Article

    Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

    Insert

    MAP3K2 (MAP3K2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF2785

    Plasmid

    #143618

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GLIS1 (GLIS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV hSyn GFP-Fxr1

    Plasmid

    #112732

    Purpose

    AAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoter

    Depositor

    Martin Beaulieu

    Article

    Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

    Insert

    Fxr1p (Fxr1 Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTWIN1-His6-Ssp-Httex1-23Q

    Plasmid

    #84349

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Use

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPPI4-Strep-SNAP-CTLA-4-His6

    Plasmid

    #223583

    Purpose

    Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Tags

    His6 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3401

    Plasmid

    #144877

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GTF2B (GTF2B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti.PGK.blast-Flag-MFF-WT

    Plasmid

    #74379

    Purpose

    Lentiviral expression vector containing flag tagged WT MFF isoform 5

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

20. ReactionC_pBIG1a_ATG7_ATG10

    Plasmid

    #190860

    Purpose

    Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG7 (ATG7 Human)
    ATG10 (ATG10 )
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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