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pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2

Plasmid

#112842

Purpose

transient/stable overexpression of the central domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

C1 domain of Catulin

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-10 [N269/73R]

Plasmid

#114558

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo-TmiR-Gb2

Plasmid

#25744

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-Puro

Plasmid

#110848

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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FRT3-Mb2-HA-mTfp1-2A (IG155)

Plasmid

#99621

Purpose

To clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2-HA-mTfp1

Expression

Mammalian

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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FRT1-Mb2YFP-2A (IG153)

Plasmid

#99619

Purpose

To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2EYFP

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT2-Mb2Tomato-2A (IG154)

Plasmid

#99620

Purpose

To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2Tomato

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT3-HA-H2B-Cerulean-2A (IG158))

Plasmid

#99624

Purpose

To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-Puro

Plasmid

#110852

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pIS1-mutant RhoA 3'UTR

Plasmid

#26090

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mutant RhoA 3'UTR (RHOA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Both miR-31 binding sites in 3’ UTR mutagenized. …

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only
pSBtet-FAP-TGN38-GFP

Plasmid

#242992

Purpose

Inducible FAP-TGN38-GFP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

dH6.2-TGN38-GFP (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and GFP

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-GFP-TGN38-FAP

Plasmid

#242993

Purpose

Inducible GFP-TGN38-FAP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

GFP-TGN38-dH6.2 (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and GFP

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-mCer3-TGN38-FAP

Plasmid

#242994

Purpose

Inducible mCer3-TGN38-FAP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

mCer3-TGN38-dH6.2 (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and mCer3

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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PATagRFP-GRP78

Plasmid

#238419

Purpose

Expresses GRP78 fused to the photoactivatable Tag-red fluorescent protein (PATagRFP-GRP78)

Depositor

Amy Shiu Lee

Article

Tseng et al Life Sci Alliance. 2019 Aug 15;2(4):e201

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

PATagRFP

Expression

Mammalian

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-Fbxw7opt

Plasmid

#241795

Purpose

Expresses Fbxw7 gene in mammalian cells.

Depositor

Diego Castrillon

Article

Cuevas et al Proc Natl Acad Sci U S A. 2019 Nov 26. p

Insert

Fbxw7 (FBXW7 Human)

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-NR5A1-FLAG

Plasmid

#242227

Purpose

Expression of full-length human SF-1 using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

NR5A1 (NR5A1 Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-ABL2(K281M)

Plasmid

#215470

Purpose

Dox-inducible lentiviral transfer vector with Arg kinase-dead (K281M)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ABL2 (ABL2 Human)

Use

Lentiviral

Mutation

K281M (kinase-inactivating)

Promoter

mCMV, Doxycycline-inducible

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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GST-GRP78-N Terminus (pGEX4T1)

Plasmid

#238422

Purpose

Expresses GRP78 N terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-C Terminus (pGEX4T1)

Plasmid

#238423

Purpose

Expresses GRP78 C Terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-PTPN11-Clover

Plasmid

#215519

Purpose

Gateway entry vector with clover-tagged Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway entry vector

Tags

Clover

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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