We narrowed to 34,443 results for: CaS;

1. pX551-miniCMV-SpCas9D10A nickase

   Plasmid

   #216735

   Purpose

   Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   T7 and mini-CMV

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1137=Hsp70Bb-Cas9[dsRed]

   Plasmid

   #149424

   Purpose

   The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   Dmel Hsp70Bb

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZ147-BvCas12b-sgRNA-scaffold

   Plasmid

   #122447

   Purpose

   Expresses sgRNA for BvCas12b in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Expression scaffold for dCas9+sgRNA

   Plasmid

   #171799

   Purpose

   PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

   Depositor

   Stephen Elledge

   Article

   Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

   Insert

   PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVCERC-5 Pvan-dcas9 (Sth3)

   Plasmid

   #133319

   Purpose

   expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus thermophilus #3)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   vanillate

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTripz Casp1-IRES-mCherry

   Plasmid

   #187294

   Purpose

   This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

   Depositor

   Alpha Yap

   Article

   Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

   Insert

   Caspase1 (Casp1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBiFC-HA-hCaspase 2 CARD-VC155

   Plasmid

   #49259

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   Caspase-2 CARD (CASP2 Human)
   

   Use

   Tags

   HA and split Venus C

   Expression

   Mammalian

   Mutation

   deleted aa 123-435

   Promoter

   CMV

   Available since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCF287_EFS-TiCas9-P2A-Puro

   Plasmid

   #211646

   Purpose

   EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   Tamoxifen-inducible SpyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAH-CTX1-rhadCas9-native

    Plasmid

    #129392

    Purpose

    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine, Histidine 840 to Alanine

    Promoter

    PrhaBAD

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-L3-Csy4-Cas9n-EGFP-L2

    Plasmid

    #141046

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-Cas9n-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CasRx gRNA targeting EZH2

    Plasmid

    #219655

    Purpose

    hU6-driven expression of CasRx guide RNA targeting EZH2. 5' processed DR followed by EZH2 guide.

    Depositor

    Weixin Tang

    Article

    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

    Insert

    CasRx gRNA targeting EZH2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyCas9 EMX1-nicking sgRNA

    Plasmid

    #169859

    Purpose

    SpyCas9 nicking sgRNA for EMX1

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SpyCas9 EMX1-nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCASP-SptP120-K55-HilA

    Plasmid

    #153329

    Purpose

    Triggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K55 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-sgfiller-eCas9-T2A-BlastR

    Plasmid

    #172437

    Purpose

    Expresses eCas9, BlastR and sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    eCas9
    BlastR
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS1: pHelper(AvCAST)_AvPSP1

    Plasmid

    #168133

    Purpose

    Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5 CASTOR2-Flag Cterm

    Plasmid

    #84490

    Purpose

    overexpression of CASTOR2 with Flag tag

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR2 (GATSL2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pSMVP-Cas9C-P2A-turboGFP

    Plasmid

    #80935

    Purpose

    Plasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBiFC-HA-hCaspase 2 FL (C303S)-VC155

    Plasmid

    #49257

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Use

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only