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pAG505-H2B-frFAST

Plasmid

#160056

Purpose

Expresses H2B-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

H2B-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120010783

Plasmid

#46009

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120010783 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pBLlSh2

Plasmid

#170981

Purpose

Expression of ShCAST under control of lacI Plac promoter/repressor pair.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Inserts

lacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pLlac01

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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NK66

Plasmid

#176606

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

Syk-BFP (Syk Mouse, Synthetic)

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHelper_O

Plasmid

#173783

Purpose

The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

186 Integrase

Use

Synthetic Biology

Expression

Bacterial

Mutation

E59G - please see depositor comments

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_W

Plasmid

#173784

Purpose

The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

Wphi Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_D

Plasmid

#173781

Purpose

The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

D145 Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 3

Plasmid

#30161

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 3

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
Id2-Halo donor

Plasmid

#113116

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Id2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGR-ECK125108723

Plasmid

#46008

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK125108723 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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p416TEF-SEC16

Plasmid

#102676

Purpose

The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

Depositor

Jens Nielsen

Article

Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

Insert

SEC16 (SEC16 Budding Yeast)

Expression

Yeast

Promoter

TEF

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pPKC1 (JCE575)

Plasmid

#89773

Purpose

yeast expression of PKC1

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC1 (PKC1 Budding Yeast)

Expression

Yeast

Promoter

yeast PKC

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-Mad2-GFP

Plasmid

#24284

Depositor

Ron Vale

Article

Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

Insert

Mad2 (mad2 Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pCS2-SP-FKBP-mCherry-TM-V5

Plasmid

#186226

Purpose

mRNA synthesis of rapalog-induced cell adhesion molecule

Depositor

Yoshihiro Morishita

Article

Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

Insert

SP-FKBP-mCherry-TM-V5

Tags

V5

Expression

Mammalian

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAF1-VP64

Plasmid

#51427

Purpose

Artificial transcription factor with VP64

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAC1820_pCR8-DR-RG6-SA-DR

Plasmid

#118654

Purpose

Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-RG6-SA-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-RGS18HIS-hphMX4

Plasmid

#26883

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

18xHis tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 1

Plasmid

#30159

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 1

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
TRPML2-CFP

Plasmid

#18831

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

CFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only