We narrowed to 7,181 results for: RAP

1. pPP537

   Plasmid

   #158803

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Use

   Tags

   2xSV40NLS-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETx-SR6v15

   Plasmid

   #176212

   Purpose

   Bacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   SR6v15
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL-p27UTR

   Plasmid

   #20881

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p27 3'UTR (Cdkn1b Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1/Puro-CAG-JEDI-1P

   Plasmid

   #202607

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDN-Sth1Cas9

   Plasmid

   #221386

   Purpose

   Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   Sth1 Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR

   Plasmid

   #176311

   Purpose

   Expresses NLS-pMag-CreC60 with tagBFP marker

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1alpha

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES

   Plasmid

   #130816

   Purpose

   Expresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   bJun-NFAST-NLS-DEVDG-mCherry-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-HA-JMJD2B

   Plasmid

   #24181

   Purpose

   Mammalian expression of human JMJD2B with HA tag

   Depositor

   Kristian Helin

   Article

   Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

   Insert

   JMJD2B (KDM4B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-FRB-VMAc-CGFP

   Plasmid

   #70226

   Purpose

   Expresses C-terminal portion of GFP along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   eGFP
   

   Use

   Tags

   FKBP-rapamycin binding (FRB) domain of the mammal…

   Expression

   Mammalian

   Mutation

   Changed Ile 129 to Cys, Deleted amino acids 1-128

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCS4333 yeTadA1.0-T7 RNAP

    Plasmid

    #137735

    Purpose

    pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

    Depositor

    Christina Smolke

    Article

    Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

    Insert

    yeTadA1.0-T7 RNAP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    E366G (See depositor comment below)

    Promoter

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PL-SIN-EOS-S(4+)-EGFP

    Plasmid

    #21317

    Purpose

    Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EOS-S(4+) promoter region

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFP

    Plasmid

    #85427

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-10
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry

    Plasmid

    #85430

    Purpose

    [Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-10 T2A PD-L1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

    Plasmid

    #169233

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-2xdBroccoli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV Puro-CCSP:GFP

    Plasmid

    #68487

    Purpose

    Retrovirus expressing GFP driven by CCSP promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Puromycin Resistance
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CCSP and LTR

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. CTB

    Plasmid

    #141166

    Purpose

    Expresses recombinant Cholera Toxin B Subunit

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    CTB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No secretion signal

    Promoter

    T7

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETSUMO-TtAgo

    Plasmid

    #210191

    Purpose

    Bacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus

    Depositor

    Phillip Zamore

    Article

    Smith et al Nat Commun. 2019 Jan 17;10(1):272. doi:

    Insert

    TtAgo (TT_P0026 Thermus thermophilus)
    

    Use

    Tags

    6x His tag and SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR_Gal4UAS_human_leucinezipperTRAIL _IRES_mCherry_PGK_tBFP

    Plasmid

    #85433

    Purpose

    Lentiviral vector for Gal4 inducible leucinezipper TRAIL with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    leucine zipper human TRAIL secreted form
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

    Plasmid

    #176310

    Purpose

    Expresses CreN59-nMag-NLS with mCherry marker.

    Depositor

    Yingxiao Wang

    Article

    Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

    Insert

    CreN59_nMag_NLS_P2A_mCherry_IRES_puroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1alpha

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEMHE-H2B-mEGFP

    Plasmid

    #105528

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    H2B (H2BC11 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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