162,597 results

1. pCW57.1_AOX-FLAG

   Plasmid

   #177984

   Purpose

   All-in-one dox-inducible expression of Alternative Oxidase

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   AOX
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA for expression in human cells

   Promoter

   TRE

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet-pLKO-puro-Scrambled

   Plasmid

   #47541

   Purpose

   Tet inducible scrambled shRNA.

   Depositor

   Charles Rudin

   Article

   Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

   Insert

   Scrambled shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Stat3-C Flag pRc/CMV

   Plasmid

   #8722

   Depositor

   Jim Darnell

   Article

   Bromberg et al Cell 1999 Aug 6;98(3):295-303.

   Insert

   Stat3-C (Stat3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   A662C and N664C. Residues changed to Cys renders…

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Stat1 alpha Flag pRc/CMV

   Plasmid

   #8691

   Depositor

   Jim Darnell

   Article

   Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

   Insert

   STAT1 alpha (STAT1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH_SBP-EGFP-GPI

   Plasmid

   #65298

   Purpose

   synchronize trafficking of EGFP-GPI from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   GPI anchor
   

   Use

   Lentiviral

   Tags

   EGFP and IL-2 Signal Sequence and Streptavidin Bi…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKL2351

   Plasmid

   #199721

   Purpose

   A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   NPTII
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEvol-MjaYRS

   Plasmid

   #153557

   Purpose

   Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli

   Depositor

   Huiwang Ai

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

   Inserts

   MJaYRS (first copy)
   MJaYRS (second copy)
   amber suppression tRNA under proK promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   glnS and pBAD

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. gRNA NIA TLS2

   Plasmid

   #196981

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs fused to TLS2 tags
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pU6-26, pU6-29

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEvolvR-enCas9-PolI3M-TBD

    Plasmid

    #113077

    Purpose

    Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI3M-TBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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