We narrowed to 12,555 results for: LIC

1. pHBS1292 TDP43 RRM-GFP G368W+W385G

   Plasmid

   #107838

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G368W and W385G mutations in LLPS reporter

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHBS1039 TDP43 RRM-GFP 2xΦ

   Plasmid

   #107806

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All hydrophobics combined to two clusters in LLPS…

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHBS1038 TDP43 RRM-GFP 4xΦ

   Plasmid

   #107805

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All hydrophobics combined to four clusters in LLP…

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHBS1037 TDP43 RRM-GFP 6xΦ

   Plasmid

   #107804

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All hydrophobics combined to six clusters in LLPS…

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGS_N_Strep

   Plasmid

   #101651

   Purpose

   Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, KpnI linker, Twin-Strep tag
   

   Use

   Tags

   Twin-Strep tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGS_C_Strep

   Plasmid

   #101652

   Purpose

   Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, MCS linker, Twin-Strep tag
   

   Use

   Tags

   Twin-Strep tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-HA-REDD1 N181-211 (#595)

   Plasmid

   #99939

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delAA181-211insNAAIRS linker

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-HA-REDD1 N187-207 (#596)

   Plasmid

   #99940

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delAA187-207insNAAIRS linker

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-HA-REDD1 85-232 (#427)

   Plasmid

   #99922

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal deletion; encodes AA 85-232

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-HA-REDD1 101-232 (#183)

    Plasmid

    #99923

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 101-232

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-HA-REDD1 1-192 (#421)

    Plasmid

    #99925

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal deletion; encodes AA 1-192

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-HA-REDD1 1-209 (#430)

    Plasmid

    #99926

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal deletion; encodes AA 1-209

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-HA-REDD1 96-110 (#189)

    Plasmid

    #99929

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Internal deletion of AA 96-110

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HA-REDD1 111-135 (#190)

    Plasmid

    #99930

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Internal deletion of AA 111-135

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-HA-REDD1 136-153 (#191)

    Plasmid

    #99931

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Internal deletion of AA 136-153

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-HA-REDD1 200-204 (#597)

    Plasmid

    #99932

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Internal deletion of AA 200-204

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-HA-REDD1 N132-145 (#433)

    Plasmid

    #99934

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delAA132-145insNAAIRS linker

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-HA-REDD1 N153-165 (#434)

    Plasmid

    #99935

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delAA153-165insNAAIRS linker

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-HA-REDD1 N179-193 (#437)

    Plasmid

    #99936

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delAA179-193insNAAIRS linker

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-HA-REDD1 N207-225 (#439)

    Plasmid

    #99938

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delAA207-225insNAAIRS linker

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-HA-REDD1 N109-119 (#432)

    Plasmid

    #99933

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delAA109-119insNAAIRS linker

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-HA-REDD1 1-165 (#187)

    Plasmid

    #99924

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal deletion; encodes AA 1-165

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMCh-664

    Plasmid

    #92252

    Purpose

    Plasmid for expression of GST-tagged human TNRC6C CED with mutated PAM2 motif in mammalian cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    EF1A

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMCh-658

    Plasmid

    #92257

    Purpose

    Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motif

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CFF-Myc

    Plasmid

    #86062

    Purpose

    A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus Myc-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Fibrocystin (Pkhd1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    contains luminal and transmembrane domains of CD8…

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. csd2_121-308/pET21a(+)

    Plasmid

    #83293

    Purpose

    csd2, construct: 121-308, pET21a(+) no-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. csd2_140-251/pET21a(+)

    Plasmid

    #83294

    Purpose

    csd2, construct:140-251, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-139 and 252-308

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. csd1_54-312/pET21a(+)

    Plasmid

    #83291

    Purpose

    csd1, construct: 54-312, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-53

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. csd2_121-308/pET28b(+)

    Plasmid

    #83292

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. csd1_125-312/pET28b(+)

    Plasmid

    #83290

    Purpose

    csd1, construct: 125-312, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-124

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSG5 N-HA SADBs shRNA resistant

    Plasmid

    #67157

    Purpose

    N-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNA

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    Brsk1 (Brsk1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…

    Promoter

    SV40 promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pBABEpuro-ARAF_D429A

    Plasmid

    #51080

    Purpose

    mammalian expression of ARAF D429A

    Depositor

    Matthew Meyerson

    Article

    Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

    Insert

    ARAF (ARAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D429A

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBabe_puro_DEST_MYNN

    Plasmid

    #45260

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    MYNN (MYNN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN81

    Plasmid

    #45293

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    deleted 81 N-terminal amino acids

    Promoter

    Available since

    May 23, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pWzl_neo_DEST_flag_SKIL

    Plasmid

    #45303

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SNON (SKIL Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaC27

    Plasmid

    #45292

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    deleted 27 c-terminal amino acids

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN149

    Plasmid

    #45294

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

38. pQTEV-C6orf60

    Plasmid

    #34813

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    C6orf60 (FAM184A Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p198 Dync1i2.F (Ex1a)

    Plasmid

    #26448

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 2 isoform F (exon 1a) (Dync1i2 Mouse)
    

    Use

    Subcloning and sequencing

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 24, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pDONR221-hSOX10-G1238A-C1240T

    Plasmid

    #24739

    Depositor

    William Pavan

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    (sex determining region Y)-box 10 (SOX10 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    G replaced with A at position 1238 and C replaced…

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

41. HsCLYBL(aa23-340)-His

    Plasmid

    #111288

    Purpose

    bacterial expression

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    human CLYBL ( aa23-340) codon optimized for bacterial expression (CLYBL Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. HM3C NaVSp1 pET24b

    Plasmid

    #128088

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

    Depositor

    Dan Minor

    Article

    Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

    Insert

    NaVSp1p
    

    Use

    Tags

    3C Cleavage, His Tag, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pJK048

    Plasmid

    #75230

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and n/a

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. p7SL-neo-TET

    Plasmid

    #51285

    Purpose

    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    7SL RNA (RN7SL2 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    7SL upstream pollII enhancer

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCMV_Cas9–superNLS

    Plasmid

    #232430

    Purpose

    Expression plasmid for the SpCas9 nuclease with superNLS

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

    Insert

    Cas9–superNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. ABE8.8-m

    Plasmid

    #136294

    Purpose

    expresses ABE8.8-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.8-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. ABE8.17-m

    Plasmid

    #136298

    Purpose

    expresses ABE8.17-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.17-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. ABE8.13-m

    Plasmid

    #136296

    Purpose

    expresses ABE8.13-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.13-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. T7opt in pCAGGS

    Plasmid

    #65974

    Purpose

    Human codon-optimized T7 polymerase

    Depositor

    Benhur Lee

    Article

    Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

    Insert

    T7-opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA C12 ecDHFR DD YFP HA

    Plasmid

    #192815

    Purpose

    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

    Depositor

    John Hulleman

    Article

    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

    Insert

    E. coli dihydrofolate reductase
    

    Use

    Tags

    enhanced yellow fluorescent protein and hemagglut…

    Expression

    Mammalian

    Mutation

    W74R/T113S/E120D/Q146L

    Promoter

    CMV

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge