We narrowed to 14,223 results for: Cas9
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Plasmid#141252PurposeConstitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterTEF1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only
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pX330-Flag-eSpCas9 (without sgRNA)
Plasmid#92354PurposeExpression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationK848A, K1003A, R1060APromoterCbhAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJA42
Plasmid#59930PurposegRNA for cleavage at rol-6(su1006) locus in C elegansDepositorInsertrol-6(su1006) gRNA
UseCRISPRPromoterU6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_DNMT3b
Plasmid#41823PurposeExpresses a guide RNA (gRNA) to target DNMT3b for genome engineeringDepositorAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW019-2
Plasmid#85614PurposeInducible dCas9 integration vector for Bacillus subtilisDepositorInsertdcas9
ExpressionBacterialPromoterPxylA (B. megaterium)Available sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
gRNA_DNMT3a-T1
Plasmid#41821PurposeExpresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineeringDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_DNMT3a-T1 (DNMT3A Human)
UseCRISPRExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
dCas9 gateway compatible
Plasmid#128322PurposedCas9 C-terminal gateway cloning vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Blast_hs_TP73
Plasmid#214691PurposeLentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73DepositorInsertdgRNA_TP73 (TP73 Human)
UseCRISPR and LentiviralAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus
Plasmid#214678PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
NM1-5S-tRNA-SgH
Plasmid#128178PurposeExpresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoterDepositorInsertgRNA cloning cassette
UseCRISPRExpressionYeastPromoter5S rRNA-tRNA(Arg)Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMZ283
Plasmid#52224PurposeExpression of sgRNA precursor with CspCI placeholder for target cloning (see comments).DepositorInsertrrk1-sgRNA
UseCRISPRExpressionYeastPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMCSG7-H588A-NmeCas9
Plasmid#71477Purposebacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-H588A-NmeCas9
Tags6HisExpressionBacterialMutationH588APromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSW21
Plasmid#86836Purposebacterial expression of anti-CRISPR protein AcrIIA4DepositorInsertacrIIA4
ExpressionBacterialPromoteraraBADAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD3-ebony
Plasmid#83380PurposeDrosophila gRNA expression plasmid targets ebonyDepositorAvailable sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BB3cH_pGAP_23*_pPFK300_Cas9
Plasmid#104912PurposehCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAct:dCas9-GCN4
Plasmid#78899PurposeExpresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell cultureDepositorInsertdCas9-10XGCN4
UseCRISPRTagsHAExpressionInsectPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti Cas9/SB100
Plasmid#155294PurposeLentiviral expression of Cas9 and SB100DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSB100X-P2A-spCas9
ExpressionMammalianAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA-Lib
Plasmid#53121PurposesgRNA expression construction in a 3rd generation lentiviral backboneDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
th-P2A-EGFP donor
Plasmid#65562PurposeIntron targeting-mediated EGFP knockin at the zebrafish th locus.DepositorAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEN243-EN1953
Plasmid#224546PurposeCas9-2A-puro and sgRNA targeting the first coding ATG of mouse NipblDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl (Nipbl Mouse)
ExpressionMammalianAvailable sinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only