We narrowed to 6,040 results for: WUS;

1. pCAGGS-FLAG-BMAL1-S519A

   Plasmid

   #186834

   Purpose

   Expresses FLAG-BMAL1-S519A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 519 to Alanine

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEos3-Rac1

   Plasmid

   #92039

   Purpose

   Expresses mEos3-Rac1 fusion protein

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Use

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 200 bp Axin2 basic

   Plasmid

   #25703

   Depositor

   Eric Fearon

   Article

   Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

   Insert

   Axin 2 promoter (AXIN2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pAcCas12nHS_RV6_PT1_empty

   Plasmid

   #203810

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6_PT1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-f:PGC1(delta CTD)

   Plasmid

   #1030

   Depositor

   Bruce Spiegelman

   Article

   Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

   Insert

   PGC1a (Ppargc1a Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   delta CTD

   Promoter

   Available since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a_HOXA11_P2A_Hygro_Barcode

   Plasmid

   #120453

   Purpose

   Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   HOXA11 (HOXA11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE2-TetOminiCMV-Oct4

   Plasmid

   #136611

   Purpose

   Dox-inducible lentiviral vector expressing mouse Oct4

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK3_020

   Plasmid

   #123735

   Purpose

   Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   ERT2-fkbp-spdCa9(205-1368)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-kanMX6-P81nmt1-GFP

   Plasmid

   #39291

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pC1300_pUB10_pco-nCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197981

    Purpose

    T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-nCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hiv7CMV-Cremyc-2A-GFP

    Plasmid

    #117148

    Purpose

    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

    Depositor

    Qiang Lu

    Article

    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

    Insert

    Cremyc-2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-PSPC1-CT

    Plasmid

    #124374

    Purpose

    expresses EGFP-PSPC1-CT131 in mammalian cell

    Depositor

    Yuh-Shan Jou

    Article

    Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

    Insert

    PSPC1 CT (PSPC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

    Plasmid

    #170248

    Purpose

    Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C124S

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. UB061 (FCK-stGtACR2-fusionRed)

    Plasmid

    #135765

    Purpose

    expresses soma targeted GtACR2

    Depositor

    Adam Cohen

    Article

    Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

    Insert

    stGtACR2
    

    Use

    Lentiviral

    Tags

    Fusion Red

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221 NLRP1 A66V

    Plasmid

    #166817

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    A66V

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PDLIM7_PDZ_1

    Plasmid

    #103940

    Purpose

    Protein expression and purification of ENIGMA PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDLIM7 (PDLIM7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ239A (dFnCas12a)-TV

    Plasmid

    #136379

    Purpose

    CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    dFnCas12a-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D917A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221 NLRP1 S1213A

    Plasmid

    #166824

    Purpose

    Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    S1213A

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Gal4 PGC1 alpha 1-350

    Plasmid

    #8893

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Gal4 DBD

    Expression

    Mammalian

    Mutation

    amino acids 1-350 of PGC-1 alpha

    Promoter

    Available since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pMXs-B4

    Plasmid

    #31708

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-92a
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only