We narrowed to 14,121 results for: CAN
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Plasmid#229553PurposeBroad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only
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mKOkappa-2A-mTurquoise2
Plasmid#98837PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmKOkappa-2A-mTurquoise2
ExpressionMammalianPromoterCMVAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM441
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBlueScript-humanAPOBEC1
Plasmid#134606PurposePlasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activityDepositorArticleAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-crRNA-Cm
Plasmid#158711PurposePlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNADepositorInserteforCP (for crRNA cloning)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterTetOAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Avr2-GFP pZK538
Plasmid#118011Purposeexpresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .DepositorArticleInsertsdspAvr2
mCherry
TagsEGFP and HDELExpressionBacterial and PlantPromoter35SAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
SUMO_MT3
Plasmid#200303PurposeBacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
ciCas9_pcDNA5
Plasmid#100550PurposeExpresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.DepositorInsertciCas9
UseCRISPR; Frt, flp-inTagsFLAGExpressionMammalianAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3XP3.RFP-tan
Plasmid#83474PurposeRFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsert3XP3.RFP-tan
UseCRISPRExpressionBacterial and InsectPromoter3XP3 eye-expression PromoterAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
MYC-NKX3.1
Plasmid#42895DepositorAvailable SinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only