We narrowed to 116,161 results for: CIL

1. pEF5/FRT-DEST EVC2ala5-TEV-YFP-Flag

   Plasmid

   #41012

   Depositor

   Rajat Rohatgi

   Article

   Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

   Insert

   Ellis van Creveld syndrome 2 (EVC2 Mouse)
   

   Use

   Tags

   TEV-YFP-Flag

   Expression

   Mammalian

   Mutation

   changed amino acids 1185-1188 to Alanine

   Promoter

   Available since

   Nov. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag

   Plasmid

   #41011

   Depositor

   Rajat Rohatgi

   Article

   Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

   Insert

   Ellis van Creveld syndrome 2 (EVC2 Mouse)
   

   Use

   Tags

   TEV-YFP-Flag

   Expression

   Mammalian

   Mutation

   changed amino acids 1183-1186 to Alanine

   Promoter

   Available since

   Nov. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

   Plasmid

   #138147

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSP6-RNAP

   Plasmid

   #48143

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase SP6
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   V314A (numbering based on NCBI Sequence ID: NP_85…

   Promoter

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

   Plasmid

   #138143

   Purpose

   Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PguCas13b
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA5 TEX264wt-cGFP

   Plasmid

   #220211

   Purpose

   Mammalian expression of wild type TEX264 fused at C-terminus to GFP

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Strep-strep-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CoV2-M-IRES-E

   Plasmid

   #177938

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-Rab11-WT

   Plasmid

   #101047

   Purpose

   Expresses HA-tagged human Rab11-WT in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

   Insert

   RAB11 (RAB11A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiRNAGuide_003

    Plasmid

    #192505

    Purpose

    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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