We narrowed to 9,406 results for: Coli
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Plasmid#178202PurposeExpression of Rok from the hns promoterDepositorInsertphns + rok
UseTagsExpressionBacterialMutationPromoterhns promoterAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD410
Plasmid#178200PurposeExpression of sRok from the hns promoterDepositorInsertphns + srok
UseTagsExpressionBacterialMutationPromoterhns promoterAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.040
Plasmid#184974PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
UseTagsExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.109
Plasmid#184978PurposeTest effect of extending a1/a2 on LYP1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationLYP1 donor E27stop, a1/a2 length extended to 27 bpPromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.107
Plasmid#184976PurposeTest effect of extending a1/a2 on CAN1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationCAN1 donor G444stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.111
Plasmid#184980PurposeTest effect of extending a1/a2 on TRP2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationTRP2 donor E64stop, a1/a2 length extended to 27 bpPromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.113
Plasmid#184982PurposeTest effect of extending a1/a2 on FAA1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationFAA1 donor P233stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.039
Plasmid#184973PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R1CiM_4EGFP_R3EGFPi2
Plasmid#185968PurposeMolecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.DepositorInsertMolecular IMPLY gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-NLSlacZ-stop-L2
Plasmid#186358PurposeEntry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ E. coli, Synthetic)
UseExpression of nuclear lacz reporterTagsNuclear localization signalExpressionMutationPromoterAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R3tdTomatoi2
Plasmid#185969PurposeExpresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.DepositorInsertPr-i2rbs-tdTomato cassette in reverse direction in Network Brick
UseSynthetic BiologyTagsExpressionMutationPromoterPRAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-T-P110A
Plasmid#180237PurposeExpresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110DepositorInsertColicin E1
UseTags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T domain of ColE1 …PromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-bhaB5
Plasmid#167812PurposeExpresses His6- and MBP-tagged BhaB5 in E. coliDepositorInsertBhaB5
UseTagsHis6, MBPExpressionBacterialMutationPromoterT7Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2343_Tier3(SB)-YPet_Blast
Plasmid#169641PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven YPet and BlastR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2344_Tier3(SB)-mRuby2_Blast
Plasmid#169642PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and BlastR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2339_Tier3(SB)-Ruby_Puro
Plasmid#169637PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2354_Tier3(PB)-Ruby_PuroR
Plasmid#169653PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro
Plasmid#169665PurposeTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGK / RPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2333_Tier3(Lenti_inverse)-YPet_Puro
Plasmid#169664PurposeTier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)DepositorInsertPRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / RPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_032
Plasmid#180528PurposeEntry vector containing pLac promoterDepositorInsertpLac promoter
UseTagsExpressionBacterialMutationPromoterAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-mTFP1-T7
Plasmid#173225PurposeEncodes mTFP fluorescent protein. To be used in cell-free systemDepositorInsertmTFP1
UseCell-free systemTagsExpressionBacterialMutationPromoterT7Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA-DAB1
Plasmid#162691PurposeExpresses DabAB1 inorganic carbon transport complexDepositorInsertDabAB1 inorganic carbon transport complex
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT-9251
Plasmid#124226PurposeBacterial SpCas9-HF1 expressionDepositorInsertSpCas9-HF1
UseCRISPRTagsExpressionBacterialMutationPromoterTetR/TetAAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
N229A Lo-MetQ
Plasmid#118268PurposeN229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structureDepositorInsertMetQ (N229A) (metQ E. coli)
UseTags10x His Tag and Enterokinase Cleavage SiteExpressionBacterialMutationChanged Asparagine (Asn) 229 to Alanine (Ala)PromoterT7 PromoterAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW120
Plasmid#115947PurposeORF insert for replicon MoCloDepositorInsertLvl0 TetR SapI/BsaI Mut
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG250
Plasmid#80582Purposerecipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptIIDepositorInsertspnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJOG251
Plasmid#80583Purposerecipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTADepositorInsertspnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)
Plasmid#75471PurposeTranscriptional unit for kanamycin resistance geneDepositorInsertNptII
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAgrobacterium tumefaciens Nopaline synthase promo…Available SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCD12_BC
Plasmid#66023PurposeMoClo Basic Part: RBS - BiCistronic Design, medium strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD12:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCD2_BC
Plasmid#66024PurposeMoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only