We narrowed to 9,393 results for: Coli

1. pET28b-MseLig1

   Plasmid

   #107071

   Purpose

   Expresses M. sedula DNA Ligase 1 in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

   Insert

   DNA ligase 1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-G2K

   Plasmid

   #70169

   Purpose

   bacterial expression of G2K (d-glycerate 2-kinase)

   Depositor

   Paul Sims, George Reed

   Article

   et al Journal of Molecular Catalysis B: Enzyma

   Insert

   garK/G2K
   

   Use

   Tags

   6xHIS and V5

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMH-HT_RcCHAD

   Plasmid

   #127782

   Purpose

   Expresses RcCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   RcCHAD
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAtLCYbSK

   Plasmid

   #53588

   Purpose

   Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

   Insert

   lcyB (LYC Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. MS2-ecDHFR

   Plasmid

   #65859

   Purpose

   Expresses RNA-binding protein MS2 with eDHFR fusion tag

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

   Inserts

   MS2
   E. coli Dihydrofolate Reductase (folA )
   

   Use

   Tags

   HA and NLS

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCDW115

   Plasmid

   #70062

   Purpose

   artificial transcription template "pause ablation"

   Depositor

   Jeff Gelles

   Article

   Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

   Insert

   lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pGEX-4T1 mouse Tankyrase1 5ARC (171–961)

   Plasmid

   #132638

   Purpose

   expresses mouse Tankyrase1 5ARC (171–961) in E. coli

   Depositor

   Wenqing Xu

   Article

   DaRosa et al Protein Sci. 2018 Jun;27(6):1057-1067. d

   Insert

   Tankyrase1 (171–961)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSETA_crE6

   Plasmid

   #172928

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorE6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19-P70a-Bottom-T

   Plasmid

   #87313

   Purpose

   Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system "Bottom" transcript
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTH07

    Plasmid

    #70063

    Purpose

    artificial transcription template "distal pause"

    Depositor

    Jeff Gelles

    Article

    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

    Insert

    lambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

11. 36INS-J23100_GB

    Plasmid

    #78669

    Purpose

    36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 36INS-J23100_FB

    Plasmid

    #78668

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 36INS-J23100_EB

    Plasmid

    #78667

    Purpose

    36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 36INS-J23100_AB

    Plasmid

    #78666

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHBFD1

    Plasmid

    #53565

    Purpose

    Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    hbfd1 cDNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRL1058

    Plasmid

    #70686

    Purpose

    Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.

    Depositor

    Peter Wolk

    Article

    Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

    Insert

    Transposon Tn5-1058
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-28a-CylA

    Plasmid

    #48198

    Purpose

    Expresses CylA (fatty acyl-AMP ligase) in E. coli

    Depositor

    Emily Balskus

    Article

    Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

    Insert

    CylA
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. T7-AglC-His6

    Plasmid

    #89726

    Purpose

    Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    AglC
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. T7-AglK-His

    Plasmid

    #89714

    Purpose

    Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    AglK
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-P70a-Top-T

    Plasmid

    #87312

    Purpose

    Expresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Top" transcript
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p70rg 1

    Plasmid

    #17828

    Depositor

    Jay Keasling

    Article

    Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

    Insert

    E. coli optimized red flourescent protein (RFPec), GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

22. pET28-p53 (Dog 1-77)

    Plasmid

    #62060

    Purpose

    Expression of Dog p53 (residues 1-77) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-77

    Promoter

    T7

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pFLIP30

    Plasmid

    #65499

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-mCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMD19T-slr0230-PL31-sgRNANT1-KmR

    Plasmid

    #73221

    Purpose

    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    PL31-sgRNA NT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PL31

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTH09

    Plasmid

    #70064

    Purpose

    artificial transcription template "terminator insertion"

    Depositor

    Jeff Gelles

    Article

    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

    Insert

    lambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pFLIP32

    Plasmid

    #65500

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFLIP39

    Plasmid

    #65507

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p70rg 5

    Plasmid

    #17829

    Depositor

    Jay Keasling

    Article

    Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

    Insert

    E. coli optimized red flourescent protein (RFPec), GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

29. pCMK83

    Plasmid

    #226793

    Purpose

    Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Insert

    slr0214 (slr0214 Synechocystis sp. PCC 6803)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tetracycline resistance cassette promoter (consti…

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 2M-T-Mce3R_K262A

    Plasmid

    #225728

    Purpose

    To express Mce3R mutant K262A in E. coli

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    mce3R
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    K262A

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28b-MelG

    Plasmid

    #225729

    Purpose

    To express MelG (Rv1937) in E. coli

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    melG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28b-VapBC35

    Plasmid

    #225733

    Purpose

    To express VapBC35(Rv1962) Toxin antitoxin in E. coli

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    vapBC35
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 2M-T_Mce3R

    Plasmid

    #225726

    Purpose

    To express Mce3R in E. coli

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    mce3R
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-PHOSPHO2-TEV-Twin-Strep

    Plasmid

    #222017

    Purpose

    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli

    Depositor

    Chiaki Murakami

    Insert

    PHOSPHO2 (PHOSPHO2 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Bacterial

    Mutation

    deleted stop codon (TAA)

    Promoter

    lac promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCN34e-yfp

    Plasmid

    #227641

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 2M-T-Mce3R_R53A

    Plasmid

    #225727

    Purpose

    To express Mce3R mutant R53A in E. coli

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    mce3R
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    R53A

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTrc-clbS_Ealbertii

    Plasmid

    #223360

    Purpose

    Encoding ClbS from Escherichia albertii CB9786

    Depositor

    Emily Balskus

    Article

    Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

    Insert

    Encoding ClbS from Escherichia albertii CB9786 (Codon optimized for E coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTrc-clbS_Frischella

    Plasmid

    #223358

    Purpose

    Encoding ClbS from Frischella perrara

    Depositor

    Emily Balskus

    Article

    Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

    Insert

    Encoding ClbS from Frischella perrara (Codon optimized for E coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTrc-clbS_Snod

    Plasmid

    #223355

    Purpose

    Encoding ClbS from Snodgrassella alvi wkB2

    Depositor

    Emily Balskus

    Article

    Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

    Insert

    Encoding ClbS from Snodgrassella alvi wkB2 (Codon optimized for E coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTrc-clbS_Mixta

    Plasmid

    #223352

    Purpose

    Encoding ClbS from Mixta theicola

    Depositor

    Emily Balskus

    Article

    Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

    Insert

    Encoding ClbS from Mixta theicola (Codon optimized for E coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge