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pcDNA3-PKCZeta KR HA Tagged

Plasmid

#24608

Depositor

Jeff Wrana

Article

Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Insert

PKCZeta (Prkcz Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

K281R mutation in the ATP binding domain causing …

Promoter

Available since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA5-FRT-TO-Nsp5

Plasmid

#157685

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp5 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3 Flag MKK7b2

Plasmid

#14623

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b2 (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MS2-HA-HsRRP6_Z

Plasmid

#148131

Purpose

Mammalian Expression of HsRRP6

Depositor

Elisa Izaurralde

Insert

HsRRP6 (EXOSC10 Human)

Use

Tags

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of is…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-P2X2-CFP

Plasmid

#22401

Depositor

Henry Lester

Article

Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

Insert

ATP-gated P2X channel 2 (P2rx2 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
ciCas9(L22)_pcDNA5

Plasmid

#100551

Purpose

Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(L22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-nAChr-Alpha6

Plasmid

#48812

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2QL1

Plasmid

#124665

Purpose

Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Use

Tags

2xStrep/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (AA)

Plasmid

#24240

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AA) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Threonine 56 to Alanine changed Serine …

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-Myc

Plasmid

#60693

Purpose

mammalian expression of Myc-tagged rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-Green Falcan0.3

Plasmid

#199704

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan0.3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Green Falcan10

Plasmid

#199707

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta2-PAmcherry1

Plasmid

#114190

Purpose

studying clustering of Beta2 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Insert

Adrenergic receptor beta 2 (ADRB2 Human)

Use

Tags

3HA, FLAG, and PAmcherry1

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp1

Plasmid

#157680

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5/FRT/TO HSPA14

Plasmid

#19461

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA14 (HSPA14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Paxillin/pcDNA3

Plasmid

#67775

Purpose

Paxilin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Paxillin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
ORF31-2xStrep pCDNA4

Plasmid

#129744

Purpose

Expresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF31 (ORF31 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

2xStrep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Use

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJA1

Plasmid

#19464

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJA1 (DNAJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

D244G

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-hMcl-1(T163A)

Plasmid

#25376

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1 (MCL1 Human)

Use

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed threonine 163 to alanine

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only