We narrowed to 3,452 results for: ttl

1. pAAV/D374Y-hPCSK9

   Plasmid

   #58379

   Purpose

   Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Insert

   proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   D374Y

   Promoter

   HCRApoE/hAAT

   Available Since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ166-SpRY

   Plasmid

   #161520

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   zSpRYCas9
   

   Use

   CRISPR; Gateway compatible zsprycas9 entry clone

   Tags

   3X FLAG, NLS and NLS

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGM190

   Plasmid

   #69614

   Purpose

   inducible gene expression in Streptomyces

   Depositor

   Günther Muth

   Article

   Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

   Inserts

   aphII
   tsr
   rep
   sso
   TipA promoter
   dso
   

   Use

   Streptomyces- e. coli shuttle vector

   Expression

   Bacterial

   Available Since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ262B-ABE8e

   Plasmid

   #161524

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   TadA8e-zSpRYCas9(D10A)
   

   Use

   CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ266E

   Plasmid

   #161521

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   zSpRYCas9(D10A)-PmCDA1-UGI
   

   Use

   CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ265E6

   Plasmid

   #213469

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cda-32aa linker-z…

   Tags

   No

   Expression

   Plant

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ265F2

   Plasmid

   #164722

   Purpose

   Gateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   A3A(Y130F)-zCas9(D10A)NG-UGI
   

   Use

   CRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)

   Plasmid

   #191650

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCK314

   Plasmid

   #110545

   Purpose

   Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

   Inserts

   Modified PrhaBAD (CRP-binding sites removed)
   yfp
   rhaS
   

   Expression

   Bacterial

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti myc-NL-GW

    Plasmid

    #113454

    Purpose

    Lentiviral NanoLuc Gateway shuttle vector for N-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Lentiviral and Luciferase

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. attB53_SNCB+_attB53

    Plasmid

    #183614

    Purpose

    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Inserts

    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    

    Use

    Rmce

    Tags

    Myc and human CD8a signal sequence

    Expression

    Mammalian

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUDP012

    Plasmid

    #101167

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ265G2

    Plasmid

    #164723

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-ziSpyMac(D10A)-UGI)
    

    Use

    CRISPR; Gateway compatible a3a/y130f-ispymaccas9-…

    Expression

    Plant

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ165

    Plasmid

    #109327

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    Promoter EC1.2 enhancer fused to EC1.1 promoter

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR221-CCL5.Pro

    Plasmid

    #79492

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    CCL5 Promoter (CCL5 Synthetic, Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    CCL5 Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ262m

    Plasmid

    #161522

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221 NLRP1 A66V

    Plasmid

    #166817

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    A66V

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR E2 HSPter-RPS5Ap-mito

    Plasmid

    #163146

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF

    Depositor

    Shin-ichi Arimura

    Article

    Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

    Insert

    A terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence
    

    Use

    Entry vector

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti GW-mCit-PA

    Plasmid

    #113457

    Purpose

    Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCitrine-ProteinA

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221 NLRP1 P1214R

    Plasmid

    #166822

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    P1214R

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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