2,172 results

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pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Tags

miRFP670

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-mRFP

Plasmid

#117781

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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TALEN1257

Plasmid

#32280

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting hey2 (hey2 Zebrafish)

Use

T7

Tags

3X FLAG and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2+-Pard3-EGFP-PIF6

Plasmid

#154914

Purpose

Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).

Depositor

Clare Buckley, Jonathan Clarke

Article

Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

Insert

par-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)

Use

Xenopus/avian/zebrafish

Tags

1-100 amino acids of Arabidopsis PIF6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-zAsCpf1

Plasmid

#107185

Purpose

plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

AsCpf1

Use

CRISPR

Tags

NLS-3xHA

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-eGFP

Plasmid

#117794

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-B-actin

Plasmid

#117808

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-CAAX-B-actin

Plasmid

#117810

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA p53, mitfa:Cas9

Plasmid

#118841

Purpose

Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

tp53 gRNA (tp53 Zebrafish)

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c

Plasmid

#107599

Purpose

An entry vector with U6a and U6c promoter driving guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Inserts

U6a promoter
U6c promoter

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

Plasmid

#58380

Purpose

tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

avi-EGFP-rpl10a

Tags

Avi Tag and EGFP

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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Danio alpha A-crystallin 1kb promoter/AcGFP

Plasmid

#98096

Purpose

aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha A-crystallin 1 kb fragment (-1028/-1)

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

Plasmid

#135766

Purpose

This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

Depositor

Nadia Mercader Huber

Article

Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

Insert

Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)

Use

Zebrafish

Promoter

UAS

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHis-zfEpoa

Plasmid

#65611

Purpose

Express soluble His-tagged zebrafish erythropoietin in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

Insert

erythropoietin (epoa Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1- Ncad-GFP

Plasmid

#35676

Depositor

James Jontes

Article

Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

Insert

Ncad (cdh2 Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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ZE11 tiggywinkle hedgehog CMV

Plasmid

#17060

Depositor

Randall Moon

Insert

tiggywinkle hedgehog (shhb Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
41 pBS119 d10L

Plasmid

#13065

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

fli genomic (fli1 Zebrafish)

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pFRT-DestFLAGHA_RNAseH1

Plasmid

#65783

Purpose

constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Zebrafish, Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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SI3-Flrt3

Plasmid

#28217

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Flrt3 mouse monoclonal antibody (flrt3 Zebrafish)

Expression

Mammalian

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pTol2-DRv7

Plasmid

#78627

Purpose

GESTALT V7 barcode for zebrafish

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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