2,162 results

1. pFRT-DestFLAGHA_RNAseH1

   Plasmid

   #65783

   Purpose

   constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Zebrafish, Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-EEA1

   Plasmid

   #109560

   Purpose

   Multisite gateway vector for 5' tagging with early endosome associated 1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   early endosome associated 1 (eea1 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+ Integrin beta 1b-mKate

   Plasmid

   #105975

   Purpose

   in vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.

   Depositor

   Caren Norden

   Article

   Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

   Insert

   Integrinbeta1b
   

   Use

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p3E-rab27a

   Plasmid

   #109685

   Purpose

   Multisite gateway vector for 3' tagging with rab27a

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   rab27a (rab27a Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Rab27a cds in pGem-T Easy

   Plasmid

   #80546

   Purpose

   Can be used for in situ hybridisation or other purposes

   Depositor

   Rob Parton

   Article

   Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

   Insert

   rab27a (rab27a Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKSII/INSeGFP

   Plasmid

   #53787

   Purpose

   1 kb zebrafish insulin promoter driving eGFP

   Depositor

   Larry Moss

   Article

   diIorio et al Dev Biol. 2002 Apr 1;244(1):75-84.

   Insert

   INS promoter/eGFP/pA
   

   Use

   Zebrafish

   Tags

   eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   zebrafish insulin/ZIP

   Available Since

   Jan. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pISceI Dusp6:memGFP

   Plasmid

   #32668

   Purpose

   Expresses membrane localized EGFP under the Dusp6 promoter

   Depositor

   Michael Tsang

   Article

   Wang et al Development. 2011 Jan;138(1):45-54. Epub

   Insert

   Dusp6 promoter (dusp6 Zebrafish)
   

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Rab33bb in pCSDEST2

   Plasmid

   #140882

   Purpose

   Expression of zebrafish GFP-tagged Rab33bb in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab33bb
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2+ Zf cdh1-deltaCyot-GFP

   Plasmid

   #185412

   Purpose

   GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   cdh1 (cdh1 Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Cytoplasmic domain deleted

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Zf Slit1a

    Plasmid

    #61279

    Purpose

    contains zebrafish Slit1a cDNA

    Depositor

    Chi-Bin Chien, Lara Hutson

    Article

    Hutson et al Dev Dyn. 2003 Nov;228(3):358-69.

    Insert

    Slit1a (slit1a Zebrafish)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

11. Zf Slit1b

    Plasmid

    #61280

    Purpose

    contains zebrafish Slit1b cDNA

    Depositor

    Chi-Bin Chien, Lara Hutson

    Article

    Hutson et al Dev Dyn. 2003 Nov;228(3):358-69.

    Insert

    Slit1b (slit1b Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pLV_PP1-mCitrine

    Plasmid

    #172472

    Purpose

    Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
    

    Use

    Lentiviral

    Tags

    mCitrine and prolactin signal sequence (cleaved p…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCK004 ubi:mCerulean_SV40 (#302)

    Plasmid

    #195978

    Purpose

    Expression construct

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubi:mCerulean
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. actc1b-mKate2-CDC42

    Plasmid

    #109531

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hsp70:mKate2-NWASP-CA

    Plasmid

    #105967

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    NWASP-CA
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ZP10 Snail

    Plasmid

    #16931

    Depositor

    Randall Moon

    Insert

    snail (snai1a Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

17. actc1b-mKate2-Bin1b

    Plasmid

    #109509

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to Bin1b

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-kcnk3a

    Plasmid

    #208985

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk3a (kcnk3a Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-kcnk3b

    Plasmid

    #208986

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk3b (kcnk3b Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDestTol2-actinb-kcnk9-IRES-EGFP

    Plasmid

    #164949

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnk9 (kcnk9 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge