2,246 results
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Plasmid#188698PurposeGateway promoter element for the zebrafish irg1 geneDepositorAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only
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p5E-1.7kbfabp6
Plasmid#159087Purposep5E with promoter sequence 1.7kb upstream of zebrafish fabp6 geneDepositorInsert1.7kb fabp6 promoter (fabp6 Zebrafish)
UsePromoter fragment 5' entry vector for tol2 c…Promoter1.7kb fabp6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3e UbB polyA
Plasmid#188702PurposeGateway 3' element encoding the ubiquitin B polyadenylation sequenceDepositorAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2+ Zf GFP-vclb
Plasmid#185411PurposeGFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMVDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertvinculin b (vclb Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf GFP-vcla
Plasmid#185410PurposeGFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMVDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertvinculin a (vcla Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato
Plasmid#172535PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistanceDepositorInsert-3.5ubb:plin2-tdtomato
MutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
hsp70l: hAPOB48-mCherry.
Plasmid#140639PurposeHeat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.DepositorUseZebrafish expressionTagsmCherryMutationAPOB48: amino acid 1-2180Promoterhsp70lAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-CDC42-DN
Plasmid#109584PurposeMultisite gateway vector for 3' tagging with dominant negative CDC42DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCDC42 (cdc42 Zebrafish)
UseZebrafish plasmidsMutationdominant negativeAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-RhoAb-DN
Plasmid#109580PurposeMultisite gateway vector for 3' tagging with dominant negative RhoAbDepositorAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-lamp1-mKate2
Plasmid#109507PurposeMuscle specific zebrafish expression of mKate2 fused to lamp1DepositorAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-mRFP
Plasmid#117793PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCre
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-gcry-mRFP
Plasmid#117778PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgal4/VP16
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-cmlc2-TagBFP
Plasmid#117780PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgal4/VP16
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-cmlc2-eGFP
Plasmid#117782PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgal4/VP16
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-cmlc2-eGFP
Plasmid#117776PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3x3 Stop sequence
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA spred1, mitfa:Cas9
Plasmid#118843PurposeTargets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertspred1 gRNA (spred1 Zebrafish)
UseCRISPR; Tol2Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pDest-Tol2 (Chien)-Krt4:Gal4VP16:tdTomato:5xUAS_MCS
Plasmid#105649PurposetdTomato expression in differentiated keratinocytes of the periderm layer and during early larval stages also in the basal layer of the epidermisDepositorInsertKrt4:Gal4VP16:tdTomato:5xUAS_MCS
ExpressionMammalianAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIG (Tol2)-tp63:ikk1KD:GFP
Plasmid#105645PurposeKinase-deleted Ikk1(alpha) expression in basal keratinocytesDepositorInserttp63:ikk1KD:GFP (chuk Zebrafish)
TagsGFPExpressionMammalianMutationp63 promoter + ikk1 (kinase domain deleted) + GFPAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only