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Plasmid#117253PurposemRNA synthesisDepositorInsertBMP2
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-XTbx5
Plasmid#117247PurposemRNA synthesis in situ hyb probesDepositorInsertTbx5 (tbx5-b Frog)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCS2(+)-XSmurf1C699A
Plasmid#117245PurposemRNA synthesisDepositorInsertSmurf1-C699A mutant
UseXenopus expressionTagsExpressionMutationmutated cysteine 699 to alaninePromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2(+)-XSmurf1
Plasmid#117244PurposemRNA synthesis in situ hyb probesDepositorInsertSmurf1
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-BRAT-EnR
Plasmid#117252PurposemRNA synthesisDepositorInsertBRAT-EnR (vegt-a Frog)
UseXenopus expressionTagsExpressionMutationchimeric protein with engrailed repressor domain…PromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSP64T-BMP4
Plasmid#117254PurposemRNA synthesisDepositorInsertBMP4
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-XTbx5-EnR
Plasmid#117249PurposemRNA synthesisDepositorInsertTbx5-EnR (tbx5-b Frog)
UseXenopus expressionTagsExpressionMutationchimeric protein with engrailed repressor domain…PromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2-R232Q
Plasmid#124064PurposeControl protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATLDepositorInsertcytXlATL2-R232Q
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2
Plasmid#124063PurposeFragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.DepositorInsertcytXIATL2
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP
Plasmid#124066PurposeE. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.DepositorInsertcytATL-R232Q-sfGFP
UseTagssfGFPExpressionBacterialMutationR232QPromotertrcAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2-6Gly-sfGFP
Plasmid#124065PurposeE. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.DepositorInsertcytATL-sfGFP
UseTagssfGFPExpressionBacterialMutationPromotertrcAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-peripherin-CTS
Plasmid#86070PurposeA bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.DepositorInsertPeripherin (prph2.S Frog)
UseTagsGSTExpressionBacterialMutationcontains the region from 317-336 of peripherinPromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
x.Amylase
Plasmid#87132PurposeTo make in situ hybridization probe for Xenopus amylase.DepositorInsertAmylase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSI HA Nup53xl
Plasmid#86000Purposemammalian expression of HA tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsHAExpressionMammalianMutationcodon optimzed for bacterial expressionPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSI myc Nup53xl
Plasmid#86001Purposemammalian expression of myc tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsmycExpressionMammalianMutationcodon optimzed for bacterial expressionPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
x.hdac8-ORF
Plasmid#87134Purposemammalian expression of hdac8DepositorInserthdac8 (hdac8.L Frog)
UseTagsExpressionMammalianMutationPromotersimian CMV IE94Available sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Xlim-3 (lhx3)
Plasmid#74139PurposePartial cDNA clone for Xlim-3 (lhx3), named LH23-1. Cloned in BS KS+. To prepare RNA, sense: HindIII/T7; antisense: BamHI/T3.DepositorInsertlhx3 (lhx3 Frog)
UseTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Xlim-1 (Lhx1) 3m
Plasmid#74136PurposeThis is a mutant of Xlim-1/Lhx1 with one point mutation in each of the two LIM domains. Cloned in XbetaM. To prepare sense RNA: SalI/SP6.DepositorInsertlhx1 3m
UseExpression in frog embryoTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only