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We narrowed to 1,212 results for: Ncl

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Showing: 421 - 450 of 1212 results

  4. pCW57-mCherry-2A-BRD4 Iso AdelCTD

    Plasmid
    #137722
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)
    Depositor
    Scott Floyd
    Article
    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi
    Insert
    BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Truncated at amino acid 1328
    Promoter
    Tight TRE promoter
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    137722-plasmid-map-sequence-id-352678

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  5. pCW57-mCherry-2A-BRD4 Iso CdelET

    Plasmid
    #137723
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)
    Depositor
    Scott Floyd
    Article
    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi
    Insert
    BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Deleted amino acids 600-682
    Promoter
    Tight TRE promoter
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    137723-plasmid-map-sequence-id-288120

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  8. pEMS1401

    Plasmid
    #29198
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available Since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29198-plasmid-map-sequence-id-13613

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  9. pEMS1538

    Plasmid
    #29261
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available Since
    Oct. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29261-plasmid-map-sequence-id-13676

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  10. pGEMHE-RnCENP-O-203-GFP

    Plasmid
    #205183
    Purpose
    Expresses chimeric rat CENP-O with mouse CENP-O middle region including central RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Michael Lampson
    Article
    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208
    Insert
    CENP-O (Cenpo, Cenpo Rat, Mouse)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Chimeric rat CENP-O with mouse CENP-O middle regi…
    Promoter
    T7
    Available Since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    205183-plasmid-map-sequence-id-409738

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  11. A1-LCD Aro--

    Plasmid
    #169716
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Available Since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169716-plasmid-map-sequence-id-334852

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  12. A1-LCD Perfect Mix

    Plasmid
    #169718
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. TYR and PHE shuffled into 5 clusters.
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Available Since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169718-plasmid-map-sequence-id-334846

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  13. A1-LCD Aro-

    Plasmid
    #169715
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Available Since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169715-plasmid-map-sequence-id-334843

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  14. A1-LCD Aro+

    Plasmid
    #169717
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. PHE and TYR amino acids shuffled to optimize mixing.
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Available Since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169717-plasmid-map-sequence-id-334791

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  16. Δ1-60MEK1C222

    Plasmid
    #164641
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C222
    Depositor
    Ben Davis
    Article
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S222C/C277S/C376S; residues 1-60 at N-terminus we…
    Promoter
    T7
    Available Since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164641-plasmid-map-sequence-id-328776

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  17. Δ1-60MEK1C218

    Plasmid
    #164643
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C218
    Depositor
    Ben Davis
    Article
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S218C/C277S/C376S; residues 1-60 at N-terminus we…
    Promoter
    T7
    Available Since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164643-plasmid-map-sequence-id-329215

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  18. Δ1-60MEK1C218C222

    Plasmid
    #164645
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C218C222
    Depositor
    Ben Davis
    Article
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S218C/S222C/C277S/C376S; residues 1-60 at N-termi…
    Promoter
    T7
    Available Since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164645-plasmid-map-sequence-id-329217

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  19. pEMS1132

    Plasmid
    #29053
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP/Cre fusion reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-Cre-NLS
    Available Since
    Jan. 9, 2012
    Availability
    Academic Institutions and Nonprofits only
    29053-plasmid-map-sequence-id-13468

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  20. lucMAPT-GenRep

    Plasmid
    #214674
    Purpose
    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214674-plasmid-map-sequence-id-424937

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  21. pMini-CMV-NLS-dead R-IscB_ADAR2dd-NES_T2A_mCherry (W98X)_U6-ωRNA

    Plasmid
    #246432
    Purpose
    All-in-one plasmid. Expresses R-IscB_ADAR2dd in mammalian cells for A-to-I RNA editing. Inactive mCherry included to assess A-to-I editing. Spacer included can direct mCherry fluorescence recovery.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID, fused to human ADAR2 deaminase domain
    mCherry
    ωRNA
    Tags
    HIV NES, SGGSSGGSSGSETPGTSESATPESSGGSSGGS linker …
    Expression
    Mammalian
    Mutation
    R-IscB: changed Aspartic Acid 60 to Alanine, chan…
    Promoter
    CMV and U6
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    246432-plasmid-map-sequence-id-490908

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  27. pUB2.1

    Plasmid
    #40728
    Purpose
    Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background
    Depositor
    Thomas Hughes
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40728-plasmid-map-sequence-id-128586

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  28. pOpen-TthRT

    Plasmid
    #165566
    Purpose
    DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Thermus thermophilus (Tth) RT
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165566-plasmid-map-sequence-id-340094

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  29. pUAST-3x attB

    Plasmid
    #220329
    Purpose
    includes 3 standard UAS cassettes
    Depositor
    Erdem Bangi
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    220329-plasmid-map-sequence-id-442491

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  30. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid
    #101893
    Purpose
    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mTagBFP2
    Promoter
    CMV immediate early
    Available Since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    101893-plasmid-map-sequence-id-211159

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Showing: 421 - 450 of 1212 results