Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 4,504 results for: pcr

Showing: 421 - 450 of 4504 results

  3. CFP-PCRD-FRB

    Plasmid
    #87449
    Purpose
    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels
    Depositor
    Xiaodong Liu
    Article
    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1
    Insert
    PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
    Use
    Tags
    6xHis-T7, CFP, and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87449-plasmid-map-sequence-id-174912

    Enlarge

  4. pAC148-pCR8-dCas9VP96

    Plasmid
    #48220
    Purpose
    dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP96 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP96
    Expression
    Mutation
    D10A;H840A
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48220-plasmid-map-sequence-id-69505

    Enlarge

  7. pCRII-Topo Couptf1

    Plasmid
    #45870
    Depositor
    Jeffrey Macklis
    Insert
    Couptf1 in situ probe (Nr2f1 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1755-2381 of NM_010151.2
    Promoter
    Available since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pAC1-pCR8-dCas9VP48

    Plasmid
    #48214
    Purpose
    dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP48 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP48
    Expression
    Mutation
    D10A;H840A
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48214-plasmid-map-sequence-id-69493

    Enlarge

  11. pCRII-Topo Lhx2

    Plasmid
    #45873
    Depositor
    Jeffrey Macklis
    Insert
    Lhx2 in situ probe (Lhx2 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1809-2217 of NM_010710.3
    Promoter
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pCRII-Topo Hnrp

    Plasmid
    #45872
    Depositor
    Jeffrey Macklis
    Insert
    Hnrnpa2b1 in situ probe (Hnrpa2b1 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1145-1370 of NM_182650.3
    Promoter
    Available since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pCR8/GW/TOPO GFP

    Plasmid
    #69798
    Purpose
    Gateway entry vector for GFP
    Depositor
    Gerard Cagney
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. pCRII-Topo Semcap3

    Plasmid
    #45874
    Depositor
    Jeffrey Macklis
    Insert
    Semcap3 in situ probe (Pdzrn3 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 3184-3805 of AF127085.1
    Promoter
    Available since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. pCRISPEY-Z3-Nat-ADE2

    Plasmid
    #232106
    Purpose
    Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    Z3
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232106-plasmid-map-sequence-id-462311

    Enlarge

  28. pCRISPEY-Z3-Hyg-ADE2

    Plasmid
    #232107
    Purpose
    Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    Z3
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232107-plasmid-map-sequence-id-462308

    Enlarge

  29. pCRISPR-cBEST-v2-SF14P

    Plasmid
    #209448
    Purpose
    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter
    Depositor
    Tilmann Weber
    Article
    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021
    Insert
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PtipA; sgRNA expression from SF14P promoter
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209448-plasmid-map-sequence-id-416625

    Enlarge

  30. pCRISPR-cBEST-v2-kasOP*

    Plasmid
    #209447
    Purpose
    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter
    Depositor
    Tilmann Weber
    Article
    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021
    Insert
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PtipA; sgRNA expression from kasOP* promoter
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209447-plasmid-map-sequence-id-416622

    Enlarge

Showing: 421 - 450 of 4504 results