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Addgene

We narrowed to 12,488 results for: BASE

Showing: 561 - 600 of 12488 results
  1. GFP-itis pBE-nt

    Plasmid
    #195343
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA
    Depositor
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    Use
    CRISPR
    Tags
    Expression
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pSLIK-Neo-TGmiR-Luc

    Plasmid
    #25745
    Purpose
    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.
    Depositor
    Insert
    Luciferase miR-shRNA
    Use
    Lentiviral and RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
  3. pSLIK-Venus-TmiR-Luc

    Plasmid
    #25739
    Purpose
    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.
    Depositor
    Insert
    Luciferase miR-shRNA
    Use
    Lentiviral and RNAi
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
  4. pSLIK-Neo-TmiR-Luc

    Plasmid
    #25743
    Purpose
    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.
    Depositor
    Insert
    Luciferase miR-shRNA
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
  5. pYPQ265N2

    Plasmid
    #174001
    Purpose
    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing
    Depositor
    Insert
    rAPOBEC1-zCas9(D10A)-SpRY-UNG
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pYPQ265O2

    Plasmid
    #174002
    Purpose
    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing
    Depositor
    Insert
    rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pYPQ265L2

    Plasmid
    #174000
    Purpose
    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing
    Depositor
    Insert
    UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. CROPseq-3P5P

    Plasmid
    #219680
    Purpose
    CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 561 - 600 of 12488 results