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Showing: 561 - 600 of 1308 results

  2. pTSara-C

    Plasmid
    #60722
    Purpose
    Expresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    Residues 180-880 of T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Only amino acids 180-880 of T7 RNAP
    Promoter
    PBAD
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60722-plasmid-map-sequence-id-97708

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  3. pTSara-N

    Plasmid
    #60721
    Purpose
    Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    Residues 1-179 of T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Only amino acids 1-179 of T7 RNAP
    Promoter
    PBAD
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60721-plasmid-map-sequence-id-97707

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  5. IBMc396

    Plasmid
    #161954
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    161954-plasmid-map-sequence-id-328230

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  8. pCH6 - pBait-ChiX

    Plasmid
    #174662
    Purpose
    arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.
    Depositor
    Katherine Berry
    Article
    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507
    Insert
    ChiX
    Use
    Tags
    1xMS2 hairpin
    Expression
    Bacterial
    Mutation
    Promoter
    pBad
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174662-plasmid-map-sequence-id-361921

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  9. IBMc071

    Plasmid
    #161943
    Purpose
    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161943-plasmid-map-sequence-id-327544

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  10. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161955-plasmid-map-sequence-id-326361

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  11. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161942-plasmid-map-sequence-id-329042

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  12. IBMc226

    Plasmid
    #161944
    Purpose
    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161944-plasmid-map-sequence-id-326341

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  16. IBMc326

    Plasmid
    #161949
    Purpose
    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161949-plasmid-map-sequence-id-327545

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  19. IBMc227

    Plasmid
    #161948
    Purpose
    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetR
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161948-plasmid-map-sequence-id-326357

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  20. pBLADE(FP6**)-mCherry

    Plasmid
    #168049
    Purpose
    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.
    Depositor
    Barbara Di Ventura
    Article
    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038
    Inserts
    Blue light-inducible AraC dimers in Escherichia coli
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101** and PBAD
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168049-plasmid-map-sequence-id-332594

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  22. pTSara

    Plasmid
    #60720
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T7 RNAP split between positions 179-180 and wt T7…
    Promoter
    PBAD
    Available since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60720-plasmid-map-sequence-id-97706

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  23. pSP019

    Plasmid
    #168195
    Purpose
    expresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.
    Depositor
    James Fleckenstein
    Insert
    eatA[H134R]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H134R mutation
    Promoter
    araBAD
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    168195-plasmid-map-sequence-id-339468

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  24. pFG018

    Plasmid
    #62817
    Purpose
    Bacterial inducible gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    62817-plasmid-map-sequence-id-114422

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  25. pKP1469

    Plasmid
    #160815
    Purpose
    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
    Depositor
    Kathleen Postle
    Insert
    exbB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160815-plasmid-map-sequence-id-316117

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  26. pX2-Cas9

    Plasmid
    #85811
    Purpose
    Arabinose inducible Cas9 in a broad host range backbone.
    Depositor
    Ryan Gill
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    85811-plasmid-map-sequence-id-177180

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  27. pCRT7 NT Topo arabinose

    Plasmid
    #73446
    Purpose
    Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides
    Depositor
    Jesse Rinehart
    Type
    Empty backbone
    Use
    Tags
    GST (N-terminal), 6xHis (C-terminal)
    Expression
    Bacterial
    Mutation
    Promoter
    pBad
    Available since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    73446-plasmid-map-sequence-id-264560

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  28. pJaM

    Plasmid
    #67966
    Purpose
    Inducible TraJ, TraM expression with arabinose.
    Depositor
    Joshua Leonard
    Insert
    TraM, TraJ
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67966-plasmid-map-sequence-id-118466

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  29. pJ

    Plasmid
    #67965
    Purpose
    Indicuble TraJ expression with arabinose.
    Depositor
    Joshua Leonard
    Insert
    TraJ
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67965-plasmid-map-sequence-id-118469

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  30. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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  31. pLaG2-T7RNAPC

    Plasmid
    #216337
    Purpose
    Arabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAP
    Depositor
    Mark Styczynski
    Insert
    LaG2-T7RNAPC fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    g2658a, g3915a
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    216337-plasmid-map-sequence-id-456751

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Showing: 561 - 600 of 1308 results