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pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

Plasmid

#187960

Purpose

AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEZYshuttle

Plasmid

#18703

Purpose

pShuttle-CMV converted Gateway Destination vector for AdEasy adenoviral vector system

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1

Plasmid

#187586

Purpose

Express FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

EWSR1 (EWSR1 Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pE-mApple L5L2

Plasmid

#187690

Purpose

C-Term Gateway cloning tag

Depositor

Mary Beckerle

Insert

mApple

Use

Gateway entry vector

Tags

mApple

Expression

Mutation

WT

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-mStrawberry

Plasmid

#187224

Purpose

Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

CRISPR

Tags

Inserted T2A-mStrawberry at 3' terminal of M…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEZY202

Plasmid

#18705

Purpose

pEG202 converted Gateway Destination vector for yeast two-hybrid bait vector

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Two-hybrid bait vector

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA-SUN2 EGFP

Plasmid

#187678

Purpose

In vivo visualization of SUN2

Depositor

Mary Beckerle

Insert

SUN2 (Sun2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES-Puro-MGMT

Plasmid

#187654

Purpose

Expresses hMGMT together with the puromycin resistance gene via an IRES.

Depositor

Robert Sobol

Article

Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

Insert

MGMT (MGMT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero11

Plasmid

#187928

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero11

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero11 (C19orf53 Human)

Use

Tags

Flag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP8m

Plasmid

#18717

Depositor

Edward Yeh

Article

Gao et al Nat Cell Biol. 2006 Oct . 8(10):1171-7.

Insert

FLAG-SENP8m (SENP8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C153A

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
EGFP-Plekhh1

Plasmid

#187367

Purpose

Expresses human Plekhh1 labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-p3-PE(1152)-N-term

Plasmid

#187181

Purpose

p3-PE2-N-term(1152) in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

Insert

PE2 from plasmid #132775

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_EPHB4

Plasmid

#187768

Purpose

MAC-tagged gene expression of human EPHB4

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHB4 (EPHB4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E S209D IRES GFP

Plasmid

#18765

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E S209D (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

S209D

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
MAC_C_RYK

Plasmid

#187790

Purpose

MAC-tagged gene expression of human RYK

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

RYK (RYK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

Plasmid

#187578

Purpose

Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

WRAP53 (WRAP53 Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTripz Casp1-IRES-mCherry

Plasmid

#187294

Purpose

This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

Depositor

Alpha Yap

Article

Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

Insert

Caspase1 (Casp1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187951

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD EGFP

Plasmid

#187267

Purpose

Lentiviral expression of shRNA targeting Cortactin

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

shRNA for Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

U6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti‐puro‐OgRNA_mdxE53

Plasmid

#187054

Purpose

SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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