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Showing: 281 - 300 of 1853 results

  2. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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  7. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166698-plasmid-map-sequence-id-329407

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  9. pDx_mScarlet3-SYFP2

    Plasmid
    #189764
    Purpose
    Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis
    Depositor
    Dorus Gadella
    Publication
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet3-SYFP2
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    189764-plasmid-map-sequence-id-375317

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  12. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid
    #189752
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Publication
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet3
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189752-plasmid-map-sequence-id-375290

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  16. cycLuc_TEVS

    Plasmid
    #119207
    Purpose
    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cyclic luciferase with TEVs cleavage site
    Use
    Luciferase
    Tags
    6xHis and AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119207-plasmid-map-sequence-id-232048

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  19. PYL1_nTEVp

    Plasmid
    #119213
    Purpose
    Expresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    split nTEVp in sufion with PYL1
    Tags
    AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119213-plasmid-map-sequence-id-232030

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  20. ABI_cTEVp

    Plasmid
    #119214
    Purpose
    Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    split cTEVp in fusion with ABI
    Tags
    Myc tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119214-plasmid-map-sequence-id-231821

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Showing: 281 - 300 of 1853 results