We narrowed to 1,582 results for: PSI-P

1. pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE

   Plasmid

   #198514

   Purpose

   Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV

   Tags

   EGFP and Rho1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIα minimal promotor (0.4kb)

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1_B1ChR2_TS_mScarlet_ER

   Plasmid

   #195191

   Purpose

   Highly K+ selective channelrhodopsin

   Depositor

   Peter Hegemann, Johannes Vierock

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Insert

   Bilabrum sp. K+ selective channelrhodopsin 2
   

   Use

   Tags

   ER-export sequence, Kir2.1 trafficking motif, and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVITRO1-102.1F10-IgE/λ

   Plasmid

   #50365

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hSyn_WiChR_TS_mScarlet_ER

   Plasmid

   #205997

   Purpose

   High efficient, K+ selective channelrhodopsin for the inhibition of neurons

   Depositor

   Peter Hegemann, Johannes Vierock

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Has Service

   AAV9

   Insert

   Wobblia Inhibitory Channelrhodopsin
   

   Use

   AAV

   Tags

   TS, mScarlet, ER

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1_WiChR_TS_mScarlet_ER

   Plasmid

   #195190

   Purpose

   High efficient, K+ selective channelrhodopsin for the inhibition of excitable cells

   Depositor

   Peter Hegemann, Johannes Vierock

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Insert

   Wobblia lunata inhibitory Channelrhodopsin
   

   Use

   Tags

   ER-export sequence, Kir2.1 trafficking motif, and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJET1.2-U6

   Plasmid

   #173157

   Purpose

   Single guide RNA cassette under U6 promoter

   Depositor

   Piotr Ziolkowski

   Article

   Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

   Insert

   sgRNA cassette under U6 promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CaMKIIa-C1C2-EYFP

   Plasmid

   #35519

   Depositor

   Karl Deisseroth

   Article

   Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

   Insert

   ChR1-ChR2 Chimera
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   June 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn_CapChR2_eYFP

   Plasmid

   #188035

   Purpose

   Expresses CapChR2-eYFP in mammalian neurons

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-eYFP
   

   Use

   AAV

   Tags

   eYFP

   Expression

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   hSyn

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn_CapChR2_mScarlet

   Plasmid

   #188039

   Purpose

   Expresses CapChR2-mScarlet in mammalian neurons

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-mScarlet
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   hSyn

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

    Plasmid

    #52494

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E90R, D156N, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPML1 (GB0045)

    Plasmid

    #68164

    Purpose

    Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtML1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDP085

    Plasmid

    #198425

    Purpose

    pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120

    Depositor

    Markus Schmid

    Article

    You et al Nat Commun. 2017 May 17;8:15120. doi: 10

    Insert

    E. coli BirA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. JW617

    Plasmid

    #64809

    Purpose

    CUC3 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC3 (CUC3 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA binding domain and Myc

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. JW624

    Plasmid

    #64808

    Purpose

    CUC2 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA binding domain

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHsp18.2 (GB0153)

    Plasmid

    #68171

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp18.2 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHsp70B (GB0155)

    Plasmid

    #68172

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp70B promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTol1-UAS:CheRiff-tdTomato

    Plasmid

    #124234

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CheRiff fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CheRiff-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTHsp18.2 (GB0035)

    Plasmid

    #68186

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp18.2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPAtUbq10 (GB0223)

    Plasmid

    #68174

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq10 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPAct2noUTR (GB0192)

    Plasmid

    #68177

    Purpose

    Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtAct2 promoter (no UTR)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge