We narrowed to 15,976 results for: nes

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pmScarlet-H_NES_C1

Plasmid

#85061

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NES-KGECO1-P2A-mGreenLantern

Plasmid

#232843

Purpose

AAV transfer plasmid for CAG-mediated co-expression of KGECO1 and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Inserts

NES-KGECO1
mGreenLantern

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NES-jRGECO1-P2A-mGreenLantern

Plasmid

#232844

Purpose

AAV transfer plasmid for CAG-mediated co-expression of jRGECO1 and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Inserts

NES-jRGECO1
mGreenLantern

Use

AAV

Tags

nuclear export sequence, 6xHis, T7, Xpress tag

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40

Plasmid

#233368

Purpose

hSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP

Plasmid

#233355

Purpose

CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP

Plasmid

#233370

Purpose

CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Use

Lentiviral

Tags

NES and mEGFP

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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H_NES 27kDa

Plasmid

#201367

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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L_NES 27kDa

Plasmid

#201368

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Adeno_NES-EGFP

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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M_NES 27kDa

Plasmid

#201366

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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p23-NES-RfxCas13d-msfGFP-NES-Flag

Plasmid

#165076

Purpose

overexpression of RfxCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RfxCas13d

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

Plasmid

#138146

Purpose

Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NES

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGEN-dPspCas13b-4EHP-NES-mCherry

Plasmid

#233610

Purpose

Expression of catalytically-inactive PspCas13b fused with 4EHP in mammalian cells

Depositor

Shintaro Iwasaki

Article

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Insert

dPspCas13b-4EHP-NES-mCherry

Use

CRISPR

Tags

3xFlag and mCherry

Mutation

H133A/H1058A

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

Plasmid

#182824

Purpose

Cre-dependent expression of cytosolic APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

APEX.NES-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pWN081

Plasmid

#233858

Purpose

MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3a-VP48-2XOaf1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NES-NS3a-NLS-VP48-2XOaf1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

Plasmid

#120555

Purpose

Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

Depositor

Yu Wang

Article

Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

Insert

Cas9, NES, ERT2 and GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pPS1372

Plasmid

#1353

Depositor

Pamela Silver

Article

Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

Insert

pADH-NLS-NES-GFP2

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pQP-2473

Plasmid

#138976

Purpose

Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD

Plasmid

#170124

Purpose

Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD-NES

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a core promoter

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA

Plasmid

#210503

Purpose

expresses LAUNCHERsbmv component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP

Plasmid

#233353

Purpose

CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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