165,025 results

1. pFUW-tetO-NFKB1

   Plasmid

   #139822

   Purpose

   doxycycline-inducible expression of Human NFKB1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   NFKB1 (NFKB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP

   Plasmid

   #179636

   Purpose

   TEVP-inducible single TM SEAP RELEASE construct

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   SEAP RELEASE
   

   Expression

   Mammalian

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.4_CEACAM6-His

   Plasmid

   #149337

   Purpose

   for eukaryotic expression and purification of CEACAM6-His6

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

   Insert

   CEACAM6 (CEACAM6 Human)
   

   Tags

   IL-2 signal peptide and polyhistidine

   Expression

   Mammalian

   Mutation

   point mutation (GGA to GCA) to replace 'G-g…

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EF1α-mNeonGreen-Z1

   Plasmid

   #135613

   Purpose

   mNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.

   Depositor

   Jordan Green

   Insert

   mNeonGreen
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a-HTLV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-SFFV-KTN1-WPRE-UbC-Emerald

   Plasmid

   #225941

   Purpose

   Lentiviral vector plasmid expressing human kinectin 1 (KNT1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva, Evan Reid

   Inserts

   KTN1 (KTN1 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

   Plasmid

   #220493

   Purpose

   To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEC2861 (pSpy1C-PgyrA_ffluc)

   Plasmid

   #218511

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PgyrA

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. dTAG NLRP1

   Plasmid

   #166825

   Purpose

   dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pP2-phiV10

   Plasmid

   #196333

   Purpose

   Plasmid expressing chimeric P2-phiV10 tail fiber

   Depositor

   Baojun Wang

   Article

   Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

   Insert

   chimeric tail fiber P2-phiV10
   

   Expression

   Bacterial

   Promoter

   P2 late promoter

   Available Since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpCas9(BB)-T2A-HygR

    Plasmid

    #118153

    Purpose

    SpCas9 expression vector

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    hSpCas9-T2A-HygR
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge