We narrowed to 14,473 results for: cas9
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Plasmid#81226PurposeCloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sitesDepositorInsertEsp31-Neo-Esp31 deletion cassette upstream of EGFP
ExpressionMammalianPromoterpCBaAvailable SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAct:MCP-VP64
Plasmid#78904PurposeExpresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertMS2:VP64
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceMarch 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWp9-1
Plasmid#73852PurposepFUGW-UBCp-RFP-CMVp-GFP-U6p-GFP-sg1DepositorInsertUBCp-RFP-CMVp-GFP-U6p-GFP-sg1
UseLentiviralAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX458_GAPDH-sgRNA
Plasmid#249575PurposeExpresses SpCas9, GFP, and sgRNA targeting GAPDH.DepositorInsertGAPDH-sgRNA
UseCRISPRPromoterU6 promoterAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGMF1-M
Plasmid#242203PurposeOsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in monocots.DepositorInsertLevel1 OsU6-2p::sgRNA1 for subcloning of 20 bp target sequence
UseCRISPR and Synthetic BiologyExpressionPlantPromoterOsU6-2pAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGMF2-M
Plasmid#242204PurposeOsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in monocots.DepositorInsertLevel1 OsU6-2p::sgRNA2 for subcloning of 20 bp target sequence
UseCRISPR and Synthetic BiologyExpressionPlantPromoterOsU6-2pAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGMF1-D
Plasmid#242205PurposeAtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in dicots.DepositorInsertLevel1 AtU6-26p::sgRNA1 for subcloning of 20 bp target sequence
UseCRISPR and Synthetic BiologyExpressionPlantPromoterAtU6-26pAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBBM
Plasmid#243675PurposeExpresses a maize optimized CDS of BABY BOOM in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminatorDepositorInsertMaize optimized CDS of BABY BOOM
ExpressionPlantAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWUS2
Plasmid#243676PurposeExpresses a maize optimized CDS of WUSCHEL2 in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminatorDepositorInsertMaize optimized CDS of WUSHCEL2
ExpressionPlantAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHYG
Plasmid#243678PurposeExpresses the HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminatorDepositorInsertHYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS
ExpressionPlantAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28(a)-p2c-eGFP
Plasmid#242758PurposeExpresses eGFP fluorophore with fused p2c targeting ligand for expression in E. coliDepositorInsertP2C-EGFP
ExpressionBacterialAvailable SinceSept. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNRCreBle
Plasmid#234431PurposePicochlorum celeri ShBle insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removalDepositorInsertCre recombinase
UseCRISPR, Cre/Lox, and Synthetic Biology ; Green al…MutationPicochlorum celeri endoglucanase intron insertionPromoterP celeri nitrate reductaseAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
Reckleen_3plus_sgRNA_ptet_pos5a
Plasmid#233461PurposeLevel 1 part, Ptet sfgfp dropout sgRNA for position 5a, for building RECKLEEN plasmid with multiple sgRNAsDepositorInsertLevel 1 part, Ptet sfgfp dropout sgRNA for position 5a, ColE1 ori, and KanR.
UseCRISPRAvailable SinceApril 24, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Reckleen_3plus_sgRNA_ptet_pos5a1
Plasmid#233462PurposeLevel 1 part, Ptet sfgfp dropout sgRNA for position 5a1, for building RECKLEEN plasmid with multiple sgRNAsDepositorInsertLevel 1 part, Ptet sfgfp dropout sgRNA for position 5a1, ColE1 ori, and KanR.
UseCRISPRAvailable SinceApril 24, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Reckleen_3plus_sgRNA_ptet_pos5a2
Plasmid#233463PurposeLevel 1 part, Ptet sfgfp dropout sgRNA for position 5a2, for building RECKLEEN plasmid with multiple sgRNAsDepositorInsertLevel 1 part, Ptet sfgfp dropout sgRNA for position 5a2, ColE1 ori, and KanR.
UseCRISPRAvailable SinceApril 24, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Reckleen_3plus_sgRNA_ptet_pos5b
Plasmid#233464PurposeLevel 1 part, Ptet sfgfp dropout sgRNA for position 5b, for building RECKLEEN plasmid with multiple sgRNAsDepositorInsertLevel 1 part, Ptet sfgfp dropout sgRNA for position 5b, ColE1 ori, and KanR.
UseCRISPRAvailable SinceApril 24, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Reckleen_3plus_sgRNA_ptet_pos5b1
Plasmid#233465PurposeLevel 1 part, Ptet sfgfp dropout sgRNA for position 5b1, for building RECKLEEN plasmid with multiple sgRNAsDepositorInsertLevel 1 part, Ptet sfgfp dropout sgRNA for position 5b1, ColE1 ori, and KanR.
UseCRISPRAvailable SinceApril 24, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Reckleen_3plus_sgRNA_ptet_pos5b2
Plasmid#233466PurposeLevel 1 part, Ptet sfgfp dropout sgRNA for position 5b2, for building RECKLEEN plasmid with multiple sgRNAsDepositorInsertLevel 1 part, Ptet sfgfp dropout sgRNA for position 5b2, ColE1 ori, and KanR.
UseCRISPRAvailable SinceApril 24, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPEY-Z3-Kan
Plasmid#232102PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Hyg
Plasmid#232101PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSKT001
Plasmid#218594PurposeExpress degron assay: Degradation tag-YFP-ePTS1DepositorInsertYFP
TagsUbiY degradation tag and ePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1357
Plasmid#218595PurposeExpress Pex11 and the degron assay: Degradation tag-YFP-ePTS1DepositorInsertPex11
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS1461
Plasmid#218587PurposeExpress peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPPDepositorInsertErg12
TagsePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1Cas-LT1
Plasmid#210556PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1pDepositorInsertpMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
ExpressionPlantAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAf-CRISPR-ctfR1
Plasmid#207992PurposeCRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavusDepositorInsertctfR1
UseCRISPRPromoterAspergillus flavus U6Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Modular BE4max for GG insertion
Plasmid#198891PurposeEasily insert deaminase of choice into BE4max using Golden Gate cloningDepositorInsertCRISPR associated protein 9 (cas9 Streptococcus pyogenes)
TagsNLSExpressionMammalianPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199487PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199488PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199489PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TC9
Plasmid#202088Purposeentry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-destDepositorInsertTevCas9 + sgRNA cassette
UseCRISPRAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCL.103
Plasmid#184988PurposeExpress Cas9-P2A-Eco1RTDepositorInsertCas9-P2A-Eco1RT
TagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.102
Plasmid#184987PurposeExpress -Eco1 RT-P2A-Cas9DepositorInsertEco1RT-P2A-Cas9
TagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.005
Plasmid#184971PurposeExpress Cas9 in yeastDepositorInsertIntegrating inducible cassette: Cas9
TagsSV40NLSExpressionYeastPromoterGal1-10Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
rtTA-sg-tdTomato166/892
Plasmid#188488PurposegRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.DepositorInserttdTomato sgRNA
UseCRISPRExpressionMammalianMutationNonePromoterU6Available SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
rtTA-sg-tdTomato90/816
Plasmid#188487PurposegRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.DepositorInserttdTomato sgRNA
UseCRISPRExpressionMammalianMutationNonePromoterU6Available SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
rtTA-sg-mGreenLantern/EGFP
Plasmid#188482PurposegRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.DepositorInsertmGreenLantern/EGFP sgRNA
UseCRISPRExpressionMammalianMutationNonePromoterU6Available SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-AtpU6-29
Plasmid#160219PurposeAtpU6-29 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-29 promoter
UseGolden gate level 0 piece to express grnasAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC4 Grin1-smFP-V5 KI
Plasmid#183439PurposeFlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)DepositorInsertgRNA and smFP V5 donor
UseCRISPRExpressionMammalianAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-tgRNA-Gal80
Plasmid#169030PurposegRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6:3Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:myc-GluA2 TKIT donor
Plasmid#169423PurposeDonor DNA for TKIT knockin of Myc to GluA2 in mouseDepositorAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only