We narrowed to 5,876 results for: nls

1. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

   Plasmid

   #84244

   Purpose

   Expresses GA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sa dCas9
   GID1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

   Plasmid

   #89874

   Purpose

   expresses DRD2-iTango component in mammalian cells for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   DRD2-V2tail-TevN-BLITz1-TetR-VP16
   

   Use

   AAV

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   short isoform of DRD2 (deletion of 242-270)

   Promoter

   Human synapsin

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. eA3A-BE3 (pRZ206)

   Plasmid

   #131315

   Purpose

   CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHEN-CoV2-nanobody-G6

   Plasmid

   #194589

   Purpose

   Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   G6 (S )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe HA human Bmf

   Plasmid

   #17239

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bmf (BMF Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

6. SECURE miniABEmax-K20A/R21A (pJUL1774)

   Plasmid

   #131312

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. hA3A-BE3 (pRZ215)

   Plasmid

   #131314

   Purpose

   CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR1163

   Plasmid

   #111099

   Purpose

   Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCD2 gRNA (FANCD2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST1374-D10A-GCN4

   Plasmid

   #113025

   Purpose

   Expresses D10A-GCN4 in mammalian cells

   Depositor

   Xingxu Huang

   Article

   Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

   Insert

   D10A-GCN4 (GCN4 Budding Yeast, S. pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMV

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pST1374-GCN4-dCas9

    Plasmid

    #113023

    Purpose

    Expresses GCN4-dCas9 in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    GCN4-dCas9 (GCN4 Budding Yeast, S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    CMV

    Available since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR1164

    Plasmid

    #111100

    Purpose

    Expresses FANCD2_-_10068140.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCD2 gRNA (FANCD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. StrKDEL-IRES-mannosidase II-mTagBFP2

    Plasmid

    #165460

    Purpose

    Expressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi

    Insert

    Partial ManII-mTag2BFP fusion (Man2a1 Mouse)
    

    Use

    Tags

    Str-KDEL and mTag2BFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. VRQR-HF1 variant

    Plasmid

    #138563

    Purpose

    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. hAID-BE3 (pRZ352)

    Plasmid

    #131316

    Purpose

    CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBP-hnRNPA2_LC

    Plasmid

    #98661

    Purpose

    Expresses MBP-tagged hnRNPA2 AA 190-341

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pST1374-dCas9-GCN4

    Plasmid

    #113026

    Purpose

    Expresses dCas9-GCN4 in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    dCas9-GCN4 (GCN4 Budding Yeast, S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A, N863A

    Promoter

    CMV

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1

    Plasmid

    #101177

    Purpose

    Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MBP-hnRNPA2_deltaLC

    Plasmid

    #98664

    Purpose

    Expresses MBP-tagged hnRNPA2 without the LC domain

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    G190Stop

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MBP-hnRNPA2_FL_D290V

    Plasmid

    #98663

    Purpose

    Expresses MBP-tagged full length hnRNPA2 with D290V

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    D290V

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. TC1464

    Plasmid

    #164881

    Purpose

    CMV-bpNLS-dCas13d-bpNLS-ADARdd, ADARdd embeded in loop1

    Depositor

    Tian Chi

    Article

    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

    Insert

    dcas13d-ADARdd
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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