We narrowed to 5,601 results for: Feng Zhang

1. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

   Plasmid

   #118158

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

   Plasmid

   #118157

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124288

   Purpose

   sgRNA against human NFATc3

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   NFATc3 nuclear factor of activated T cells (NFATC3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

4. pX330-U6-BRD4-HaloTag-Guide-hspCas9

   Plasmid

   #228576

   Purpose

   Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

   Depositor

   Marcin Imielinski

   Insert

   BRD4 gRNA (BRD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GGDestX2-Amp

   Plasmid

   #157654

   Purpose

   Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NFATc4-CRISPR-gRNA#exon5-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124290

   Purpose

   sgRNA against huiman NFATc4

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   NFATc4 nuclear factor of activated T cells (NFATC4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

7. Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124283

   Purpose

   sgRNA against human Thy-1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

8. Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124285

   Purpose

   sgRNA against human Thy-1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

9. Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124284

   Purpose

   sgRNA against human Thy-1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

10. NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124289

    Purpose

    sgRNA against human NFATc4

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    NFATc4 nuclear factor of activated T cells (NFATC4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

11. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid

    #118159

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid

    #118160

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZW1-FCS-circRNA

    Plasmid

    #73449

    Purpose

    Expression of circular RNA

    Depositor

    Ling-Ling Chen

    Article

    Zhang et al Cell. 2014 Sep 25;159(1):134-47. doi: 10

    Insert

    POLR2A (POLR2A Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330 p53

    Plasmid

    #59910

    Purpose

    pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting p53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330 Pten

    Plasmid

    #59909

    Purpose

    pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting Pten (Pten Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330-sgR26

    Plasmid

    #127376

    Purpose

    Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCRISPR v2 sgKEAP1-2

    Plasmid

    #186459

    Purpose

    knock out KEAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiCRISPR v2 sgNRF2-3

    Plasmid

    #186838

    Purpose

    knock out NRF2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiCRISPR v2 sgKEAP1-3

    Plasmid

    #186837

    Purpose

    knock out KEAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KAT5 sgRNA1

    Plasmid

    #138187

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT5

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT5 (KAT5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. KAT5 sgRNA2

    Plasmid

    #138188

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT5

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT5 (KAT5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. lentiCRISPR v2 sgNRF2-4

    Plasmid

    #186839

    Purpose

    knock out NRF2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330-sgAAVS1

    Plasmid

    #85802

    Purpose

    Cas9/CRISPR vector for cut at human AAVS1 gene intron1

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX330 Ctnnb1.1

    Plasmid

    #59911

    Purpose

    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. KAT2A sgRNA2

    Plasmid

    #138186

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT2A (KAT2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. KAT2A sgRNA1

    Plasmid

    #138185

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT2A (KAT2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. lentiCRISPR v2-sgNCKAP1-2

    Plasmid

    #210134

    Purpose

    knock out NCKAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Nck-associated protein 1 (NCKAP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lentiCRISPR v2-sgNCKAP1-1

    Plasmid

    #210133

    Purpose

    knock out NCKAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Nck-associated protein 1 (NCKAP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lentiCRISPR v2-sgCOQ2

    Plasmid

    #186025

    Purpose

    knock out COQ2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Coenzyme Q2, Polyprenyltransferase (COQ2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lentiCRISPR v2-sgWAVE2-2

    Plasmid

    #210136

    Purpose

    knock out WAVE2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Actin-binding protein WASF2 (WASF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge