We narrowed to 14,145 results for: TIM
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Plasmid#76714Purpose3rd generation lentiviral gRNA plasmid targeting human PIK3C2BDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)
Plasmid#133789PurposeCAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized CjeCas9
TagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-EGFP_opt
Plasmid#106334PurposeGateway Entry vector for EGFP (codon optimized for efficient gene synthesis)DepositorInsertEGFP
UseGateway entry vectorExpressionBacterial and MammalianMutationcodon optimized for gene synthesis without affect…Available SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
TK1 gRNA (BRDN0001145069)
Plasmid#76424Purpose3rd generation lentiviral gRNA plasmid targeting human TK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NUAK1 gRNA (BRDN0001162598)
Plasmid#75577Purpose3rd generation lentiviral gRNA plasmid targeting human NUAK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001146004)
Plasmid#80174Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shJUND puro
Plasmid#58698PurposeLentiviral shRNA vector for knockdown of human JUNDDepositorAvailable SinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a(ICOS)
Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mKate2
Plasmid#199249PurposeFluorescent labelling of bacterial cells with mKate2DepositorInsertCodon-optimized mKate2 gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001146586)
Plasmid#77947Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NUAK1 gRNA (BRDN0001147733)
Plasmid#75576Purpose3rd generation lentiviral gRNA plasmid targeting human NUAK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NUAK1 gRNA (BRDN0001162525)
Plasmid#75578Purpose3rd generation lentiviral gRNA plasmid targeting human NUAK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001149423)
Plasmid#80255Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD80-mGFP
Plasmid#223591PurposeLentiviral expression of human CD80 with mGFP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PHKG1 gRNA (BRDN0001144981)
Plasmid#76356Purpose3rd generation lentiviral gRNA plasmid targeting human PHKG1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PFKFB4 gRNA (BRDN0001149495)
Plasmid#77123Purpose3rd generation lentiviral gRNA plasmid targeting human PFKFB4DepositorAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK17A gRNA (BRDN0001149480)
Plasmid#77916Purpose3rd generation lentiviral gRNA plasmid targeting human STK17ADepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK17B gRNA (BRDN0001146764)
Plasmid#76798Purpose3rd generation lentiviral gRNA plasmid targeting human STK17BDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001146792)
Plasmid#77948Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001146747)
Plasmid#77949Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001487150)
Plasmid#77950Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMMDS DEST
Plasmid#206264PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.DepositorTypeEmpty backboneUseCre-donor, multimate/gateway dest vector (multiba…Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG ChR2 E123T T159C 2A tDimer
Plasmid#85399PurposeHigh efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoterDepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVExpressionMammalianMutationE123T , T159CPromoterCAG and ribosomal skip sequence 2AAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUC2::GNSI (NrsR)
Plasmid#121537PurposeThis plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-deSpCas9-VP64-6xHis
Plasmid#92117PurposeExpression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive eSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, H840A, K848A, K1003A, R1060APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)
Plasmid#134331PurposeCAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tagDepositorInserthuman codon optimized Nme2Cas9
TagsNLS(SV40)-3xFLAGExpressionMammalianPromoterCAGAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.iGluSnFr.WPRE.SV40
Plasmid#98932PurposeCre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVExpressionMammalianPromoterCAGAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)
Plasmid#115142PurposeCAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized Mb3Cas12a
TagsNLS(nucleoplasmin) and 3xHAExpressionMammalianAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-LynG
Plasmid#206867PurposeExpression of LynG probe - LynAnchor-eGFPDepositorInsertLynG
UseRetroviralTagseGFPExpressionMammalianMutationContains Lyn amino acids 1-16Available SinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Full human ELL2
Plasmid#127253PurposeExpresses full-length human ELL2 in mammalian cellsDepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only