We narrowed to 21,629 results for: ESC

1. LifeAct-mGreenLantern

   Plasmid

   #164459

   Purpose

   Localization of mGreenLantern fluorescent protein to actin

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSII-EF-miRFP670v1-hGem(1/110)

   Plasmid

   #80006

   Purpose

   hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   hGeminin (1/110) (GMNN Human)
   

   Use

   Lentiviral

   Tags

   miRFP670v1

   Expression

   Mutation

   Promoter

   EF-1 alpha

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSII-EF-miRFP709-hCdt(1/100)

   Plasmid

   #80007

   Purpose

   hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   hCdt1(1/100) (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   miRFP709

   Expression

   Mutation

   Promoter

   EF-1 alpha

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEosEM-E_Lamin A

   Plasmid

   #226338

   Purpose

   Photoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expression

   Depositor

   Pingyong Xu

   Article

   Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

   Insert

   Lamin A (LMNA Human)
   

   Use

   Tags

   mEosEM-E

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDuEx2-NIR-GECO1

   Plasmid

   #113680

   Purpose

   Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NIR-GECO1
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mito-mGreenLantern

   Plasmid

   #164461

   Purpose

   Localization of mGreenLantern fluorescent protein to mitochondria

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   Human COX8a first 29 amino acids

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDual-eGFP(Y203)

   Plasmid

   #63559

   Purpose

   Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(Y203)
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Changed threonine at position 203 with respect to…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

   Plasmid

   #113898

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCST-AausFP1

   Plasmid

   #129499

   Purpose

   Expresses AausFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. single polypeptide FPX biosensor for calcium ion

    Plasmid

    #60887

    Purpose

    FPX biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for calcium ion
    

    Use

    Tags

    HindIII site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

    Plasmid

    #113899

    Purpose

    Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    TetR-HA-NLS-P2A-BSD-Myc
    

    Use

    Lentiviral

    Tags

    HA-Myc

    Expression

    Mutation

    Promoter

    CMV-MIE

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDisplay-GACh2.0

    Plasmid

    #106073

    Purpose

    express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

    Insert

    GPCR activation based ACh sensor (GACh) 2.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE

    Plasmid

    #218879

    Purpose

    AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2(no bind)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102G R168P

    Promoter

    Synapsin

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ER-mGreenLantern

    Plasmid

    #164463

    Purpose

    Localization and retention of mGreenLantern fluorescent protein in the ER

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-K-GECO1

    Plasmid

    #105865

    Purpose

    Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

    Depositor

    Robert Campbell

    Article

    Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

    Insert

    K-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB_NEmut

    Plasmid

    #123310

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE control sensor GRAB_NEmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LiOn-CAG∞IRFP

    Plasmid

    #154017

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    IRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. iOn-CAG∞RFP

    Plasmid

    #154014

    Purpose

    Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMM643

    Plasmid

    #112533

    Purpose

    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    luxCDABE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A17P

    Promoter

    J23107, PL(HrtO), and ProD

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC18T-mini-Tn7T-Gm-Pc-mCherry

    Plasmid

    #199247

    Purpose

    Fluorescent labelling of bacterial cells with mCherry

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized mCherry gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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