We narrowed to 7,345 results for: Lif;
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Plasmid#191097PurposeTo express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-vsfGFP-0
UseAAVTagsGreen fluorescent protein bound to enhancer nanob…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Flrt2-myc
Plasmid#72192PurposeExpress full-length Flrt2with a C-terminal myc tagDepositorInsertFlrt2 (Flrt2 Mouse)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX317-CELF4
Plasmid#115445PurposeConstitutive lentiviral expressionDepositorInsertCELF4 (CELF4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-DDR2-V5/HIS
Plasmid#201101Purposeexpression of human DDR2 receptor tyrosine kinase in mammalian cellsDepositorInserthuman DDR2 receptor tyrosine kinase, full length, wildtype (DDR2 Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-gcry-TagBFP
Plasmid#117777PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS
Plasmid#145027PurposeLentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear markerDepositorInsert3xnls-Tq-Ca-FLITS
UseLentiviralTags3xnls-Tq-Ca-FLITS and H2B-MaroonExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP
Plasmid#188773PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-NR2F2
Plasmid#192900PurposeBarcoded piggybac transposon vector with Dox-inducible expression of NR2F2DepositorInsertNR2F2 (NR2F2 Human)
UseTagsExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-CERT(PHD)(W33A)
Plasmid#221761PurposeStable expression of GFP-CERT(PHD)(W33A) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–116, mutated 33W to 33APromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-mScarlet
Plasmid#191098PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-mScarlet
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationOptimized to human codon usagePromoterCMV enhancer and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl-XL-s2193
Plasmid#166749PurposeExpress mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.DepositorInsertBcl-XL (BCL2L1 Human)
UseTagsmCerulean3ExpressionMammalianMutationPromoterHuman ferritinAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-NR4A1
Plasmid#192925PurposeBarcoded piggybac transposon vector with Dox-inducible expression of NR4A1DepositorInsertNR4A1 (NR4A1 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
kanMX6-ins2
Plasmid#195039PurposepFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertKanR
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…ExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF RBP-FL P53
Plasmid#174042PurposeExpress Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)DepositorInsertP53 gene (TP53 Human)
UseTagsThermoanaerobacter Tencongenesis ribose binding p…ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-CD8-Nb127D01-GFP
Plasmid#171577PurposeExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembraneDepositorInsertCD8-Nb127D01-GFP (CXCR2 Human)
UseTagsCD8 and GFPExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiMPH v2 (Neo)
Plasmid#92065PurposeModified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).DepositorInsertMS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
UseLentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSpB-HaloTag-p63DH
Plasmid#176116PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-gcry-eGFP
Plasmid#117779PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188765PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterSFFVAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only