We narrowed to 9,681 results for: SUB;

1. MCP-mCherry-TIAL1

   Plasmid

   #207669

   Purpose

   To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIAL1

   Depositor

   Christine Mayr

   Article

   Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

   Insert

   TIAL1 (TIAL1 Human)
   

   Tags

   MCP-mCherry

   Expression

   Mammalian

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYFP-NEAT1m_v1-RT

   Plasmid

   #97089

   Purpose

   Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   NEAT1m_v1-RT (NEAT1 Human)
   

   Use

   CRISPR

   Tags

   EYFP

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHS1184

   Plasmid

   #205965

   Purpose

   NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   DinG HNH
   

   Tags

   14xHis-TwinStrep-bdSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

   Plasmid

   #197067

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   MitoTrap-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYFP-NEAT1_IS_RT

   Plasmid

   #97091

   Purpose

   Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   NEAT1_IS_RT (NEAT1 Human)
   

   Use

   CRISPR

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pACT2-μ3B

   Plasmid

   #198175

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-3 μ3B
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAluYNF1

   Plasmid

   #50933

   Purpose

   Expresses dimeric AluY RNA in mammalian cells.

   Depositor

   Katharina Strub

   Article

   Ivanova et al Nucleic Acids Res. 2015 Mar 11;43(5):287

   Insert

   AluYNF1
   

   Expression

   Mammalian

   Promoter

   7SL RNA promoter (pol III)

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

   Plasmid

   #134863

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAMP4 (SERP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SAC1C389SdeltaTMD-FKBP-mCherry

   Plasmid

   #108124

   Purpose

   Recruitable cytosolic inactive SAC1

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   SACM1L(C389S; 1-520):FKBP1A(3-108):mCherry (SACM1L Human)
   

   Expression

   Mammalian

   Mutation

   SACM1L(C389S; 1-520)

   Promoter

   CMV

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ub[9]-mRFP[1]

    Plasmid

    #20249

    Depositor

    Nico Dantuma

    Article

    Salomons et al Mol Cell Biol. 2009 Apr;29(7):1774-85. d

    Insert

    ubiquitin C (UBC Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    changed Stop codon 686 to Glycine

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGBW-m4136458

    Plasmid

    #149531

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 13, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pILGFP95

    Plasmid

    #83550

    Purpose

    Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    PDA1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    PDA1

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pQE60 R196M wgMDH

    Plasmid

    #204273

    Purpose

    Bacterial expression of R196M wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196M

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQE60 D193E wgMDH

    Plasmid

    #204269

    Purpose

    Bacterial expression of D193E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D193E

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETDuet RagA(D130N)-RagC

    Plasmid

    #99642

    Purpose

    Bacterial expression of RagA(D130N)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D130N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFH6

    Plasmid

    #86555

    Purpose

    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    U6-26p::sgRNA scaffold
    

    Use

    CRISPR; Subcloning

    Promoter

    Arabidopsis U6-26 promoter

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETDuet RagA(T21N)-RagC

    Plasmid

    #99644

    Purpose

    Bacterial expression of RagA(T21N)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    T21N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-PAK2 wt

    Plasmid

    #31671

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 (PAK2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

19. hLOXL2-S300P

    Plasmid

    #200061

    Purpose

    LOXL2 mutant

    Depositor

    Lakshmi Santhanam

    Article

    Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

    Insert

    human lysyl oxidase like 2 (LOXL2 Human)
    

    Expression

    Mammalian

    Mutation

    S300P mutation

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS Galectin1

    Plasmid

    #19216

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Galectin1 (LGALS1 Chicken)
    

    Expression

    Bacterial

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only