We narrowed to 4,337 results for: U6 gRNA

1. pORANGE GFP-Nlgn3 KI

   Plasmid

   #131501

   Purpose

   Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Nlgn3, Nlgn3 Mouse, Rat)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE GFP-Syt7 KI

   Plasmid

   #131488

   Purpose

   Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Syt7, Syt7 Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE GFP-CADPS KI

   Plasmid

   #131482

   Purpose

   Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cadps Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330-PITCh-ACTB

   Plasmid

   #207748

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of ACTB (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. POLR2A-N CRISPR pX330

   Plasmid

   #124495

   Purpose

   A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

   Depositor

   Masato Kanemaki

   Article

   Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

   Insert

   POLR2A targeting CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORANGE CACNG8-GFP KI #2

   Plasmid

   #131474

   Purpose

   Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cacng8 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BLADE-182

   Plasmid

   #134914

   Purpose

   sgRNA targeting GFP to be used in nanoblade system

   Depositor

   Philippe Mangeot, Emiliano Ricci, Théophile Ohlmann

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Insert

   GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. plenti-px330-CRBN-T1-pGK-Pur

   Plasmid

   #107382

   Purpose

   Mammalian expression CRISPR/Cas9

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   sgRNA targeting CRBN (CRBN Human)
   

   Use

   Lentiviral; Crispr

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV ORANGE Gria1-HaloTag KI

   Plasmid

   #139655

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and HaloTag donor
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE GFP-Clta KI

    Plasmid

    #131483

    Purpose

    Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Clta Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE Shank1-GFP KI

    Plasmid

    #131500

    Purpose

    Endogenous tagging of Shank1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Shank1, Shank1 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLC-BFP-FADD

    Plasmid

    #75166

    Purpose

    LentiCRISPR-BFP with sgRNA targeting human FADD

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    FADD sgRNA (FADD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORANGE Rims2-GFP KI

    Plasmid

    #131495

    Purpose

    Endogenous tagging of RIM2: C-terminal (amino acid position: S1551)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rims2, Rims2 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. plenti-px330-CRBN-T2-pGK-Pur

    Plasmid

    #107383

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORANGE Shank2-GFP KI

    Plasmid

    #131496

    Purpose

    Endogenous tagging of Shank2: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Shank2, Shank2 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pORANGE Doc2A-GFP KI

    Plasmid

    #131478

    Purpose

    Endogenous tagging of Doc2a: C-terminal (amino acid position: L402)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Doc2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDECKO_mCherry_non-targeting

    Plasmid

    #140109

    Purpose

    CRISPR-Cas9 library validation (negative control)

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiGuide-Puro-IFITM3g1 (BB15)

    Plasmid

    #139460

    Purpose

    Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLC-RFP657-CASP8

    Plasmid

    #75164

    Purpose

    LentiCRISPR-RFP657 with sgRNA targeting human Caspase-8

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    CASP8 sgRNA (CASP8 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiGuide-Puro-NCOA7g1 (BB22)

    Plasmid

    #139457

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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