We narrowed to 5,364 results for: puc

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pSA_0_mKate2_synCoTC

Plasmid

#199469

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mKate2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pZH1 Dual G-less cassette

Plasmid

#231645

Purpose

plasmid containing dual G-less cassette for in vitro transcription

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-GFP

Plasmid

#86441

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

mCherry-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

mCherry-sfGFP

Promoter

Plac

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEXPYR

Plasmid

#70030

Purpose

High yield protein secretion in Aspergillus nidulans

Depositor

Rolf Prade

Article

Segato et al Enzyme Microb Technol. 2012 Jul 15;51(2)

Type

Empty backbone

Use

Synthetic Biology

Promoter

A. niger glucoamylase promoter (glaAp)4

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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LI D-E GFP + linker c3

Plasmid

#54410

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + GFP

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMT1002

Plasmid

#8621

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

barnase, barstar

Tags

as pMT416

Expression

Bacterial

Mutation

CI157 lambda repressor gene has two HindIII sites…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pVC_CRISPR_Cat

Plasmid

#120368

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

CRISPR_Cat

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGGF005

Plasmid

#48846

Purpose

Provides a hygromycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pUBQ10::HygromycinR::tOCS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAC37

Plasmid

#111618

Purpose

Fluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosa

Depositor

Tim Tolker-Nielsen

Article

Yang et al Microbiology. 2007 May;153(Pt 5):1318-28

Insert

PpqsA-gfp(ASV)

Expression

Bacterial

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSA_2_mKate2_synCoTC

Plasmid

#199471

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mKate2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_2_mTagBFP2_synCoTC

Plasmid

#199474

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_0_mTagBFP2_synCoTC

Plasmid

#199472

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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KIF5A_Halo_C_allele

Plasmid

#178163

Purpose

Donor vector for endogenous tagging of human KIF5A at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (KIF5A Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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HNRNPA2B1_Halo_C_allele

Plasmid

#178126

Purpose

Donor vector for endogenous tagging of human HNRNPA2B1 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (HNRNPA2B1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRE57-Ter

Plasmid

#170635

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, Ter cassette

Expression

Bacterial

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-non-targeting (pJK5)

Plasmid

#229052

Purpose

Cloning backbone for PspCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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DNMT1_Halo_C_allele

Plasmid

#178157

Purpose

Donor vector for endogenous tagging of human DNMT1 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (DNMT1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAK005

Plasmid

#128218

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 5

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 5

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAK004

Plasmid

#128217

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 4

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 4

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK006

Plasmid

#128219

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 6

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 6

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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