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1M-Mx-FLAG

Plasmid

#206941

Purpose

Mammalian Expression of 1M-Mx-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, MxEnc

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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1M-Tm-BC2

Plasmid

#206943

Purpose

Mamamlian Expression of 1M-Tm-BC2Tag EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, TmEnc

Tags

BC2

Expression

Mammalian

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_1

Plasmid

#103927

Purpose

Protein expression and purification of PDZK1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_4

Plasmid

#103945

Purpose

Protein expression and purification of PDZK1 PDZ4 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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TJP2_PDZ_3

Plasmid

#103949

Purpose

Protein expression and purification of X104 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP2 (TJP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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TJP2_PDZ_2

Plasmid

#103955

Purpose

Protein expression and purification of X104 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP2 (TJP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_2

Plasmid

#103943

Purpose

Protein expression and purification of PDZK1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_3

Plasmid

#103944

Purpose

Protein expression and purification of PDZK1 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5 WT

Plasmid

#13050

Depositor

Richard Pagano

Article

Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

Insert

RAB5A (Canis familiaris) (RAB5A Canis familiaris)

Tags

DsRed2 and HA

Expression

Mammalian

Mutation

asparagine 3 changed to serine (neutral); serine …

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-RAPH1ΔTBS

Plasmid

#194859

Purpose

Express eGFP-RAPH1 lacking the TBS domain (RAPH1 aa 2-92 deleted) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

RAPH1 with amino acids 2-92 deleted

Promoter

CMV

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL1029

Plasmid

#225600

Purpose

The STU backbone vector for tomato genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6

Plasmid

#197967

Purpose

Mammalian cell expression of wt 3xFLAG-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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p21 promoter A

Plasmid

#16462

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pCAGGS-C-TEV-Twin-Strep

Plasmid

#202527

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHDE-35S-Cas9-mCherry-UBQ

Plasmid

#78932

Purpose

For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs

Depositor

Yunde Zhao

Article

Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCSC-ASCL1-IRES-GFP-T2A-Sox11

Plasmid

#182233

Purpose

To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

Depositor

Chun-Li Zhang

Article

Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

Insert

ASCL1-IRES-GFP-T2A-Sox11

Use

Lentiviral

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-hMCP-mScarlet-I-NLS

Plasmid

#174878

Purpose

PiggyBac transposon vector for expression of MCP-mScarlet-I-NLS

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

MCP-mScarlet-I-NLS

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_CMV_N_Flag_BIRC6

Plasmid

#197973

Purpose

Mammalian cell expression of wt FLAG-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-SENP2 (catalytic domain)

Plasmid

#16357

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP2 catalytic domain (SENP2 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 365 to 590 catalytic domain of human S…

Available Since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

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