We narrowed to 3,249 results for: cat.3

1. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

   Plasmid

   #220494

   Purpose

   To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   sgRNA targeting LIG4 exon 3 (LIG4 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA4TO-3XFLAG Halo R99A nsp1

   Plasmid

   #175422

   Purpose

   Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 R99A nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS_m-AKAR5

   Plasmid

   #114139

   Purpose

   Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   AKAR5 C-terminally tagged by KRas C tail
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS_cyt-AKAR5

   Plasmid

   #114138

   Purpose

   Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   AKAR5 with C-terminal nuclear export signal (NES)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR3-vsv-GCN4-dCEN-INCENP

   Plasmid

   #108510

   Purpose

   expression of vsv-GCN4-dCEN-INCENP

   Depositor

   Susanne Lens

   Article

   van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

   Insert

   GCN4-dCEN-INCENP (INCENP Human)
   

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   replace CEN box for GCN4 dimerazation domain

   Promoter

   CMV

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR3-vsv-Incenp 8KQ

   Plasmid

   #108501

   Purpose

   expression of vsv-INCENP 8KQ

   Depositor

   Susanne Lens

   Article

   van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

   Insert

   INCENP (INCENP Human)
   

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-Nkx2-1*/Neo

   Plasmid

   #31272

   Depositor

   Monte Winslow, Tyler Jacks

   Article

   Winslow et al Nature. 2011 May 5. 473(7345):101-4.

   Insert

   Nkx2-1* (Nkx2-1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   A shNkx2-1 insensitive Nkx2-1 cDNA was created by…

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-mEGFP-hCTNNA1_DM1-3_H0-ABD+

   Plasmid

   #234554

   Purpose

   CTNNA1 M-domain deletion mutant with enhanced actin-binding domain mutations

   Depositor

   Cara Gottardi

   Article

   Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

   Insert

   mEGFP:hCTNNA1_DM-domain-H0-ABD+ (CTNNA1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Promoter

   CMV

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cdk13_intron3_sg3_pX458

   Plasmid

   #127338

   Purpose

   sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #3

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Mouse Cdk13 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6 Promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28 mOct4-POU

    Plasmid

    #206393

    Purpose

    Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    POU DNA binding domain only

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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