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C1-F-tractin-mCitrine

Plasmid

#155217

Purpose

Monitor global F-actin distribution

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Tags

mCitrine

Expression

Mammalian

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Publication

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOSV812

Plasmid

#126605

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV809

Plasmid

#126602

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV804

Plasmid

#126597

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV806

Plasmid

#126606

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1814_pCR8-gSMN2-DN2

Plasmid

#118647

Purpose

Transient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gSMN2-DN2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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E2-Crimson-FKBP(L,V)

Plasmid

#85000

Purpose

Expresses E2-Crimson-FKBP(L,V) in yeast

Depositor

Benjamin Glick

Publication

Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

Insert

E2-Crimson-FKBP(LV)

Expression

Yeast

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Skp2 WT

Plasmid

#81117

Purpose

bacterial expression plasmid of Skp2

Depositor

Sung Hee Baek

Publication

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only
pIS1

Plasmid

#74887

Purpose

targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C

Depositor

Klaus Foerstemann

Publication

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Inserts

Act5C upstream homology region
Act5C downstream homology

Tags

eGFP

Expression

Insect

Available since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

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pZE31ms2

Plasmid

#25860

Depositor

Philippe Cluzel

Publication

Le et al Proc Natl Acad Sci U S A. 2005 Jun 28. 1

Insert

ms2 binding site (x2)

Expression

Bacterial

Mutation

none

Available since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only

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p7VP1

Plasmid

#24726

Depositor

Christopher Buck

Publication

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VP1 from Human Polyomavirus 7

Expression

Mammalian

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-EF1alpha-VP64-dCas9-VP64_Blast

Plasmid

#192650

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHybrid-Electra1

Plasmid

#179474

Purpose

Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra1
mScarlet-I

Expression

Bacterial and Mammalian

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2plus

Plasmid

#164226

Purpose

Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.

Depositor

Silvia Cardona

Publication

Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

Type

Empty backbone

Expression

Bacterial

Promoter

Rhamnose-inducible

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-HA-LMW-FGF2

Plasmid

#157663

Purpose

Expresses FGF2 in mammalian cell

Depositor

Byung Il Lee

Publication

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

HA tag

Expression

Mammalian

Mutation

deleted amino acid 1-134

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMaCTag-Z07

Plasmid

#124790

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMaCTag-Z34

Plasmid

#120062

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P22

Plasmid

#120033

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Publication

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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