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pBbA6c_PsulA_sfGFP

Plasmid

#224587

Purpose

SOS response reporter

Depositor

Ophelia Venturelli

Article

Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PsulA

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Vega

Plasmid

#246323

Purpose

Photostable genetically encoded voltage indicator for imaging in HEK293T

Depositor

Peng Zou

Article

Cao et al bioRxiv 2025.05.29.656768

Insert

Vega

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-SpyTag_Δpvc10

Plasmid

#243740

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SpyTag - deleted spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SpyTag_Δpvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBR322-sgRNA-pvc18-20

Plasmid

#243747

Purpose

PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

vegfa sgRNA-pvc18-20

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-pvc10-Cas9

Plasmid

#243755

Purpose

For affinity purification of Pvc10 fused to Cas9

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc10-Cas9

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFPC3-mSufu

Plasmid

#65431

Purpose

Expresses mouse Sufu protein in mammalian cells

Depositor

Aimin Liu

Article

Zeng et al PLoS One. 2010 Dec 29;5(12):e15900. doi:

Insert

Suppressor of Fused (Sufu Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
HC-M

Plasmid

#173481

Purpose

An rrnB P1-based GFP ATP reporter in E. coli

Depositor

Rahul Sarpeshkar

Article

Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

Insert

GFP-mut2

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

rrnB P1

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBE48

Plasmid

#174656

Purpose

CAPTURE method BAC origin of replication receiver plasmid

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pB1H1

Plasmid

#12611

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Dorsal RHR in pB1H1 (dl Fly)

Tags

flag

Expression

Bacterial

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pBE44

Plasmid

#174654

Purpose

CAPTURE method 15A origin of replication receiver plasmid

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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coel::GFP

Plasmid

#8937

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Tags

GFP

Expression

Worm

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pGGY003

Plasmid

#48867

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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phU1-CasRx-non-targeting (pKW82)

Plasmid

#229050

Purpose

Cloning backbone for CasRx crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGGZ003

Plasmid

#48869

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGZ001

Plasmid

#48868

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTec_mCherry

Plasmid

#229145

Purpose

Promoter strength evaluation of Ptec

Depositor

Shrikrishnan Sankaran

Article

Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

Insert

mCherry

Expression

Bacterial

Mutation

No

Promoter

Ptec

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-CasRx-non-targeting (pEZ18)

Plasmid

#229049

Purpose

Cloning backbone for CasRx crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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phU1-PspCas13b-non-targeting (pEZ36)

Plasmid

#229053

Purpose

Cloning backbone for PspCas13b crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET30-RT1306

Plasmid

#131521

Purpose

Expresses engineered RT-1306 with His-tag

Depositor

Bryan Dickinson

Article

Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

Insert

RT-1306

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pB006

Plasmid

#182320

Purpose

It's an empty replication plasmid backbone in Bacteroides

Depositor

Lei Dai

Article

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pICH41421

Plasmid

#50339

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pB1H2

Plasmid

#12613

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

dorsal RHR in pB1H2 (dl Fly)

Tags

flag

Expression

Bacterial

Available Since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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pGGF007

Plasmid

#48847

Purpose

Provides a kanamycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::KanamycinR::tNOS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL30003

Plasmid

#50304

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p5E-lyzC

Plasmid

#107591

Purpose

An entry vector with a lyzC promoter

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

Lysozyme C promoter

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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PEG-Actinin-M-sstFRET

Plasmid

#61100

Purpose

Expression of α-actinin with internal sstFRET inserted to measure cytoskeletal stress in vivo

Depositor

Fred Sachs

Article

Meng et al J Cell Sci. 2011 Jan 15;124(Pt 2):261-9.

Insert

α-actinin-sstFRET

Expression

Mammalian

Mutation

sstFRET inserted at position 300

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pBE50

Plasmid

#174657

Purpose

CAPTURE method receiver plasmid for Bacillus cloning and heterologous expression

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-Vector-Dendra2

Plasmid

#97101

Purpose

empty vector for overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Type

Empty backbone

Use

Zebrafish

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn-pOUT

Plasmid

#213905

Purpose

Mariner transposon with outward-facing pJ23104 promoter

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and outward-facine pJ23104 promoter

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pICH41414

Plasmid

#50337

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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