We narrowed to 5,882 results for: pcDNA 3.1

1. Galphai3-mTurquoise2

   Plasmid

   #69622

   Purpose

   The heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Insert

   Galphai3-mTurquoise2
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmEGFP-C1-Nir3-LNS2

   Plasmid

   #220087

   Purpose

   C-terminal PA binding domain of Nir3

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM2 (PITPNM2 Human)
   

   Use

   Tags

   mEGFP-GGGGSHM

   Expression

   Mammalian

   Mutation

   amino acids 925-1209

   Promoter

   CMV

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCerulean-hCOL1A1

   Plasmid

   #140112

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cenp-B DBD INCENP GFP

   Plasmid

   #45237

   Purpose

   Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B-DBD, INCENP-Δcen, GFP
   

   Use

   Tags

   DNA binding domain of CENP-B and GFP

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-ShadowY-kCAAX

   Plasmid

   #115564

   Purpose

   dark ITRACK acceptor construct for dual color imaging

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   ShadowY with KRas membrane targeting domain
   

   Use

   Tags

   ShadowY

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mSIRT3-L

   Plasmid

   #33309

   Depositor

   Michael Sack

   Article

   Bao et al J Cell Biochem. 2010 May;110(1):238-47.

   Insert

   Sirt3 (Sirt3 Mouse)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TAp63gamma-(3X)HA

   Plasmid

   #26977

   Depositor

   David Sidransky

   Article

   Upadhyay et al Biochem Biophys Res Commun. 2007 May 18.

   Insert

   TAp63gamma (TP63 Human)
   

   Use

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFLAG-ZYG11A

   Plasmid

   #110551

   Purpose

   expresses a FLAG-tagged human ZYG11A protein in mamalian cells

   Depositor

   Edward Kipreos

   Article

   Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

   Insert

   ZYG11A (ZYG11A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. ER2-hfYFP

   Plasmid

   #186518

   Purpose

   Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2365-Tier1-PhCMV-TetR

    Plasmid

    #169566

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven tetracycline repressor protein class B from transposon Tn10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVH607-Tier1-PCREm4-Nluc

    Plasmid

    #169594

    Purpose

    Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Galphai1-mTurquoise2

    Plasmid

    #69620

    Purpose

    The heterotrimeric G-protein subunit, Gαi isoform 1, tagged with mTurquoise2

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Insert

    Galphai1-mTurquoise2
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pAL101

    Plasmid

    #22474

    Purpose

    Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. F-Cdk8

    Plasmid

    #15366

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Conaway et al Trends Biochem Sci. 2005 May . 30(5):250

    Insert

    CDK8 (CDK8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mito-LSSmGFP

    Plasmid

    #186536

    Purpose

    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Use

    Tags

    Human COX8a first 29 amino acids

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Histone H2B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45216

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    Histone H2B-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ER2-mGreenLantern

    Plasmid

    #186548

    Purpose

    Localization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    mGreenLantern
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ER2-LSSmGFP

    Plasmid

    #186538

    Purpose

    Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tCRMod-GGSGGS9

    Plasmid

    #111762

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS9-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Use

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. untargeted Plk1 FRET sensor Myt1 substrate

    Plasmid

    #45217

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    untargeted Plk1 FRET sensor Myt1 substrate
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CENP-B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45214

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pmiRFP670nano3-NbGFP

    Plasmid

    #184686

    Purpose

    Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3-NbGFP
    

    Use

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV2-hNCOA6:V5

    Plasmid

    #63585

    Purpose

    A subunit of the Trithorax-related (Trr) methyltransferase complex.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

    Insert

    NCOA6 (NCOA6 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

26. mSIRT3-S

    Plasmid

    #33310

    Depositor

    Michael Sack

    Article

    Bao et al J Cell Biochem. 2010 May;110(1):238-47.

    Insert

    Sirt3 (Sirt3 Mouse)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

27. optGluCl alpha EYFP

    Plasmid

    #15104

    Depositor

    Henry Lester

    Article

    Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

    Insert

    GluCl alpha (glc-1 Nematode)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HA-EcPPK1

    Plasmid

    #159279

    Purpose

    Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

    Depositor

    Michael Downey

    Article

    Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

    Insert

    HA-tagged Polyphosphate Kinase 1
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CMV-2xFLAG-Atoh1

    Plasmid

    #101734

    Purpose

    Expresses mouse Atoh1 with FLAG tag

    Depositor

    Albert Edge

    Article

    Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNIR-FbGFPY58/C-JIP

    Plasmid

    #184684

    Purpose

    NIR-Fb-based JNK inhibitor

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbGFPY58/C-JIP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge